Cloned (Comment) | Organism |
---|---|
mutant enzyme C59A is expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
mutant enzyme C59A in complex with glutathionylspermidine | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
C59A | active site mutant | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathionylspermidine + H2O | Escherichia coli | - |
glutathione + spermidine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0AES0 | bifunctional enzyme glutathionylspermidine synthetase/amidase | - |
Purification (Comment) | Organism |
---|---|
nickel affinity column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathionylspermidine + H2O | - |
Escherichia coli | glutathione + spermidine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Glutathionylspermidine synthetase/amidase | bifunctional enzyme | Escherichia coli |
GSP amidase | - |
Escherichia coli |
GspA | glutathionylspermidine amidase domain of GspSA | Escherichia coli |
GspSA | bifunctional enzyme | Escherichia coli |