Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.1.77 extracted from

  • Chiang, C.J.; Chern, J.T.; Wang, J.Y.; Chao, Y.P.
    Facile immobilization of evolved agrobacterium radiobacter carbamoylase with high thermal and oxidative stability (2008), J. Agric. Food Chem., 56, 6348-6354.
    View publication on PubMed
Search for more literature for 3.5.1.77

Application

Application Comment Organism
biotechnology immobilization of the evolved carbamoylase offers a promising way for the efficient production of D-hydroxyphenylglycine from D-L-hydroxyphenylhydantoin Agrobacterium tumefaciens

Cloned(Commentary)

Cloned (Comment) Organism
the plasmids pET20bl and pET-Chi are used for expression of the protein in Escherichia coli cells Agrobacterium tumefaciens

Protein Variants

Protein Variants Comment Organism
G75S/V237A mutant, thermal stability 55.6%, wild-type 3.0%, oxidative stability 42.8%, wild-type 5.0% Agrobacterium tumefaciens
I286V/F287A mutant, thermal stability 4.5%, wild-type 3.0%, oxidative stability 4.8%, wild-type 5.0% Agrobacterium tumefaciens
V237A/C279S mutant, thermal stability 22.4%, wild-type 3.0%, oxidative stability 31.2%, wild-type 5.0% Agrobacterium tumefaciens
V40A/G75S/V237A mutant, thermal stability 68.4%, wild-type 3.0%, oxidative stability 80.3%, wild-type 5.0% Agrobacterium tumefaciens
V40A/G75S/V237A/I286V/F287A mutant, thermal stability 72.3%, wild-type 3.0%, oxidative stability 83.1%, wild-type 5.0% Agrobacterium tumefaciens

Organism

Organism UniProt Comment Textmining
Agrobacterium tumefaciens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using metal affinity resins, immobilization on chitin beads Agrobacterium tumefaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-carbamoyl-D-p-hydroxyphenylglycine + H2O
-
 Agrobacterium tumefaciens D-p-hydroxyphenylglycine + NH3 + CO2
-
 ?

Synonyms

Synonyms Comment Organism
carbamoylase
-
 Agrobacterium tumefaciens
N-carbamoyl-D-amino acid amidohydrolase
-
 Agrobacterium tumefaciens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Agrobacterium tumefaciens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 80 relative enzyme activity 30-25%, 70°C 100% Agrobacterium tumefaciens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Agrobacterium tumefaciens

pH Range

pH Minimum pH Maximum Comment Organism
6 9 relative enzyme activity 80-30%, pH 7.0 100% Agrobacterium tumefaciens