Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Pseudomonas putida | |
2,2'-dipyridyl | - |
Pseudomonas putida | |
5,5'-dithiobis(2-nitrobenzoic acid) | weak | Pseudomonas putida | |
Ag2SO4 | - |
Pseudomonas putida | |
HgCl2 | - |
Pseudomonas putida | |
iodoacetate | weak | Pseudomonas putida | |
N-bromosuccinimide | - |
Pseudomonas putida | |
NEM | - |
Pseudomonas putida | |
PCMB | - |
Pseudomonas putida | |
phenylhydrazine | - |
Pseudomonas putida | |
Semicarbazide | - |
Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.31 | - |
N-acetyl-2-phenylethylamine | - |
Pseudomonas putida | |
0.34 | - |
N-acetylserotonin | - |
Pseudomonas putida | |
1.5 | - |
Melatonin | - |
Pseudomonas putida | |
1.6 | - |
N-Acetyl-3-phenylpropylamine | - |
Pseudomonas putida | |
5 | - |
N-acetyldopamine | - |
Pseudomonas putida | |
8.9 | - |
(R)-N-acetyl-1-methyl-3-phenylpropylamine | - |
Pseudomonas putida | |
41 | - |
N-Acetylbenzylamine | - |
Pseudomonas putida |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Pseudomonas putida | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CdCl2 | activates | Pseudomonas putida | |
CoCl2 | activates | Pseudomonas putida | |
CuSO4 | activates | Pseudomonas putida | |
MgCl2 | activates | Pseudomonas putida | |
MnCl2 | activates | Pseudomonas putida | |
NiCl2 | activates | Pseudomonas putida | |
ZnSO4 | activates | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
4 * 42000, SDS-PAGE | Pseudomonas putida |
42630 | - |
4 * 42630, SDS-PAGE | Pseudomonas putida |
150000 | - |
gel filtration | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Pseudomonas putida Sc2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture medium | - |
Pseudomonas putida | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.25 | - |
- |
Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-N-acetyl-1-methyl-3-phenylpropylamine + H2O | - |
Pseudomonas putida | (S)-1-methyl-3-phenylpropylamine + acetate | - |
? | |
Acetic acid 2-phenylethyl ester + H2O | - |
Pseudomonas putida | ? | - |
? | |
Acetic acid 3-phenylpropyl ester + H2O | - |
Pseudomonas putida | ? | - |
? | |
Melatonin + H2O | - |
Pseudomonas putida | ? | - |
? | |
additional information | no activity with acetanilide derivatives | Pseudomonas putida | ? | - |
? | |
additional information | no activity with acetanilide derivatives | Pseudomonas putida Sc2 | ? | - |
? | |
N-Acetyl-1-methyl-3-phenylpropylamine + H2O | (R)-isomer | Pseudomonas putida | (S)-1-Methyl-3-phenylpropylamine + acetate | - |
? | |
N-Acetyl-1-methyl-3-phenylpropylamine + H2O | (R)-isomer | Pseudomonas putida Sc2 | (S)-1-Methyl-3-phenylpropylamine + acetate | - |
? | |
N-Acetyl-2-phenylethylamine + H2O | - |
Pseudomonas putida | 2-Phenylethylamine + acetate | - |
? | |
N-Acetyl-2-phenylethylamine + H2O | - |
Pseudomonas putida Sc2 | 2-Phenylethylamine + acetate | - |
? | |
N-Acetyl-3-phenylpropylamine | - |
Pseudomonas putida | ? | - |
? | |
N-Acetyl-3-phenylpropylamine | - |
Pseudomonas putida Sc2 | ? | - |
? | |
N-Acetylbenzylamine + H2O | - |
Pseudomonas putida | ? | - |
? | |
N-Acetylbenzylamine + H2O | - |
Pseudomonas putida Sc2 | ? | - |
? | |
N-acetyldopamine + H2O | - |
Pseudomonas putida | ? | - |
? | |
N-Acetylserotonin + H2O | - |
Pseudomonas putida | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 42000, SDS-PAGE | Pseudomonas putida |
tetramer | 4 * 42630, SDS-PAGE | Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Pseudomonas putida |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 75 | 55°C: about 45% of maximal activity, 75°C: about 20% of maximal activity | Pseudomonas putida |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
pH 7.0, stable below | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
with N-acetyl-2-phenylethylamine as substrate | Pseudomonas putida |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | pH 6.0: about 50% of maximal activity, pH 10: about 40% of maximal activity, with N-acetyl-2-phenylethalamine as substrate | Pseudomonas putida |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
30°C, 30 min, most stable under alkaline conditions | Pseudomonas putida |