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Literature summary for 3.5.1.74 extracted from

  • Kawamoto, K.; Horibe, I.; Uchida, K.
    Purification and characterization of a new hydrolase for conjugated bile acids, chenodeoxycholyltaurine hydrolase, from Bacteroides vulgatus (1989), J. Biochem., 106, 1049-1053.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol 0.1-1 mM: slight activation, 10 mM: high activation Phocaeicola vulgatus

Inhibitors

Inhibitors Comment Organism Structure
dithiodipyridine 0.1 mM: 15.9% inhibition, 1 mM: 50.2% inhibition, 10 mM: complete inhibition Phocaeicola vulgatus
N-ethylmaleimide 0.1 mM: 3.5% inhibition, 1 mM: 27.4% inhibition, 10 mM: 90.8% inhibition Phocaeicola vulgatus
p-hydroxymercuribenzoate 0.00001 mM: 4% inhibition, 0.0001 mM: 60% inhibition, 0.001 mM: complete inhibition Phocaeicola vulgatus
sulfhydryl reagents
-
Phocaeicola vulgatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetic properties Phocaeicola vulgatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
4 * 36000, SDS-PAGE Phocaeicola vulgatus
140000
-
gel filtration Phocaeicola vulgatus

Organism

Organism UniProt Comment Textmining
Phocaeicola vulgatus
-
isolated from human feces
-
Phocaeicola vulgatus VI-31
-
isolated from human feces
-

Purification (Commentary)

Purification (Comment) Organism
strain VI-31 Phocaeicola vulgatus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.11
-
substrate: tauro-7beta-cholic acid Phocaeicola vulgatus
0.69
-
substrate: taurocholic acid Phocaeicola vulgatus
5.86
-
substrate: taurohyocholic acid Phocaeicola vulgatus
7.56
-
substrate: taurodeoxycholic acid Phocaeicola vulgatus
8.74
-
substrate: taurohyodeoxycholic acid Phocaeicola vulgatus
13.68
-
substrate: tauroursodeoxycholic acid Phocaeicola vulgatus
15.15
-
substrate: tauro-beta-muricholic acid Phocaeicola vulgatus
24.45
-
substrate: taurochenodeoxycholic acid Phocaeicola vulgatus

Storage Stability

Storage Stability Organism
-20°C, stable for 1 month without any loss of activity Phocaeicola vulgatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate specificity Phocaeicola vulgatus ?
-
?
additional information hydrolyzes taurine-conjugated bile acids, glycine-conjugated bile acids are no substrates Phocaeicola vulgatus ?
-
?
additional information no activity with glycocholic acid, glyco-7beta-cholic acid, glycodeoxycholic acid, glycochenodeoxycholic acid, glyco-beta-muricholic acid, glycoursodeoxycholic acid, glycohyocholic acid, glycohyodeoxycholic acid Phocaeicola vulgatus ?
-
?
additional information specificity for not only the amino acid moiety but also the steroidal moiety Phocaeicola vulgatus ?
-
?
additional information substrate specificity Phocaeicola vulgatus VI-31 ?
-
?
additional information hydrolyzes taurine-conjugated bile acids, glycine-conjugated bile acids are no substrates Phocaeicola vulgatus VI-31 ?
-
?
additional information no activity with glycocholic acid, glyco-7beta-cholic acid, glycodeoxycholic acid, glycochenodeoxycholic acid, glyco-beta-muricholic acid, glycoursodeoxycholic acid, glycohyocholic acid, glycohyodeoxycholic acid Phocaeicola vulgatus VI-31 ?
-
?
additional information specificity for not only the amino acid moiety but also the steroidal moiety Phocaeicola vulgatus VI-31 ?
-
?
tauro (3alpha,5beta,7beta,12alpha)-3,7,12-trihydroxycholan-24-oic amide + H2O 0.5% of activity compared to taurochenodeoxycholic acid Phocaeicola vulgatus taurine + (3alpha,5beta,7beta,12alpha)-3,7,12-trihydroxycholan-24-oic acid
-
?
tauro-beta-muricholic acid + H2O 62% of activity compared to taurochenodeoxycholic acid Phocaeicola vulgatus taurine + beta-muricholic acid
-
?
taurochenodeoxycholic acid + H2O best substrate Phocaeicola vulgatus chenodeoxycholate + taurine
-
?
taurochenodeoxycholic acid + H2O best substrate Phocaeicola vulgatus VI-31 chenodeoxycholate + taurine
-
?
taurocholic acid + H2O 2.8% of activity compared to taurochenodeoxycholic acid Phocaeicola vulgatus taurine + cholic acid
-
?
taurodeoxycholic acid + H2O 31% of activity compared to taurochenodeoxycholic acid Phocaeicola vulgatus taurine + deoxycholic acid
-
?
taurohyocholic acid + H2O 24% of activity compared to taurochenodeoxycholic acid Phocaeicola vulgatus taurine + hyocholic acid
-
?
taurohyodeoxycholic acid + H2O 36% of activity compared to taurochenodeoxycholic acid Phocaeicola vulgatus taurine + hyodeoxycholic acid
-
?
tauroursodeoxycholic acid + H2O 56% of activity compared to taurochenodeoxycholic acid Phocaeicola vulgatus taurine + ursodeoxycholic acid
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 36000, SDS-PAGE Phocaeicola vulgatus

Synonyms

Synonyms Comment Organism
CTH
-
Phocaeicola vulgatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Phocaeicola vulgatus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
48 h stable at Phocaeicola vulgatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6 6.4 broad pH-optimum Phocaeicola vulgatus