Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | no effect | Bacillus subtilis | |
2-mercaptoethanol | slight activation | Bacillus cereus | |
dithiothreitol | no effect | Bacillus subtilis | |
dithiothreitol | slight activation | Bacillus cereus |
General Stability | Organism |
---|---|
Ca2+ decreases thermostability | Bacillus subtilis |
Mg2+ decreases thermostability | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | - |
Bacillus cereus | |
iodoacetamide | no inhibition | Bacillus subtilis | |
leupeptin | peptide inhibitor | Bacillus cereus | |
leupeptin | peptide inhibitor | Bacillus subtilis | |
additional information | no inhibition by EDTA | Bacillus cereus | |
additional information | no inhibition by EDTA; strain 168, no inhibition by lysozyme, 2 mg/ml, or cell wall lysate | Bacillus subtilis | |
N-benzoyl-D-Arg-4-nitroanilide | substrate inhibition at concentration above 1 mM | Bacillus cereus | |
N-benzoyl-D-Arg-4-nitroanilide | substrate inhibition at concentration above 0.5 mM | Bacillus subtilis | |
p-chloromercuribenzene sulfonic acid | 80% inhibition | Bacillus cereus | |
p-chloromercuribenzene sulfonic acid | no inhibition | Bacillus subtilis | |
p-chloromercuribenzoate | - |
Bacillus cereus | |
p-chloromercuribenzoate | no inhibition | Bacillus subtilis | |
tosyl-L-Arg methyl ester | - |
Bacillus cereus | |
tosyl-L-Arg methyl ester | - |
Bacillus subtilis | |
tosyl-L-Lys chloromethyl ketone | - |
Bacillus cereus | |
tosyl-L-Lys chloromethyl ketone | - |
Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.029 | - |
N-benzoyl-D-Arg 4-nitroanilide | strain 168 | Bacillus subtilis | |
0.14 | - |
N-benzoyl-D-Arg 4-nitroanilide | - |
Bacillus cereus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | 80-90% in soluble form in cytoplasm | Bacillus subtilis | 5737 | - |
cytoplasm | 80-90% in soluble form in cytoplasm | Bacillus cereus | 5737 | - |
intracellular | - |
Bacillus subtilis | 5622 | - |
intracellular | - |
Bacillus cereus | 5622 | - |
additional information | strain 168, subcellular distribution, no activity in cell wall lysate and membranes | Bacillus subtilis | - |
- |
protoplast | - |
Bacillus subtilis | - |
- |
protoplast | - |
Bacillus cereus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | activation | Bacillus subtilis | |
CaCl2 | activation | Bacillus cereus | |
CaCl2 | optimum concentration: 1 mM | Bacillus cereus | |
CaCl2 | strain 168, optimal concentration: 5 mM | Bacillus subtilis | |
MgCl2 | activation, optimal concentration: 5 mM | Bacillus subtilis | |
MgCl2 | activation, optimal concentration: 5 mM | Bacillus cereus | |
NaCl | 100 mM: two- to threefold activation | Bacillus subtilis | |
NaCl | 100 mM: two- to threefold activation | Bacillus cereus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
gel filtration and PAGE | Bacillus subtilis |
66000 | - |
gel filtration and PAGE | Bacillus cereus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillaceae | - |
found in all aerobic and anaerobic members of the family tested | - |
Bacillus cereus | - |
T | - |
Bacillus cereus T | - |
T | - |
Bacillus subtilis | - |
168 trpC2 and nonspore-forming mutant 43.4 spo0A43 leu-8 | - |
Bacillus thuringiensis | - |
var. israelensis | - |
Clostridium sp. | - |
- |
- |
Lysinibacillus sphaericus | - |
2362 | - |
Lysinibacillus sphaericus 2362 | - |
2362 | - |
marine aerobic sporeforming bacterium 6A10 | - |
- |
- |
no activity in Acetobacter xylinum | - |
- |
- |
no activity in Corynebacterium bovis | - |
- |
- |
no activity in Escherichia coli | - |
- |
- |
no activity in Escherichia coli JM83 | - |
- |
- |
no activity in Streptococcus mutans serotype C | - |
- |
- |
no activity in Streptococcus pneumoniae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Bacillus cereus |
strain 168, partial | Bacillus subtilis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | enzyme is more active in vegetative cells than in germinating spores | Bacillus subtilis | - |
additional information | enzyme is more active in vegetative cells than in germinating spores | Lysinibacillus sphaericus | - |
additional information | enzyme is more active in vegetative cells than in germinating spores | Bacillus cereus | - |
additional information | enzyme is more active in vegetative cells than in germinating spores | Clostridium sp. | - |
additional information | enzyme is more active in vegetative cells than in germinating spores | Bacillaceae | - |
additional information | enzyme is more active in vegetative cells than in germinating spores | Bacillus thuringiensis | - |
additional information | enzyme is more active in vegetative cells than in germinating spores | marine aerobic sporeforming bacterium 6A10 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Bacillus subtilis |
additional information | - |
- |
Bacillus cereus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | ability to cleave N-benzoyl-D-Arg-4-nitroanilide, i.e. D-BAPA is suggested as an additional chemotaxonomic characteristic of sporeforming bacteria of the genera Bacillus and Clostridium | Bacillus subtilis | ? | - |
? | |
additional information | ability to cleave N-benzoyl-D-Arg-4-nitroanilide, i.e. D-BAPA is suggested as an additional chemotaxonomic characteristic of sporeforming bacteria of the genera Bacillus and Clostridium | Lysinibacillus sphaericus | ? | - |
? | |
additional information | ability to cleave N-benzoyl-D-Arg-4-nitroanilide, i.e. D-BAPA is suggested as an additional chemotaxonomic characteristic of sporeforming bacteria of the genera Bacillus and Clostridium | Bacillus cereus | ? | - |
? | |
additional information | ability to cleave N-benzoyl-D-Arg-4-nitroanilide, i.e. D-BAPA is suggested as an additional chemotaxonomic characteristic of sporeforming bacteria of the genera Bacillus and Clostridium | Clostridium sp. | ? | - |
? | |
additional information | ability to cleave N-benzoyl-D-Arg-4-nitroanilide, i.e. D-BAPA is suggested as an additional chemotaxonomic characteristic of sporeforming bacteria of the genera Bacillus and Clostridium | Bacillaceae | ? | - |
? | |
additional information | ability to cleave N-benzoyl-D-Arg-4-nitroanilide, i.e. D-BAPA is suggested as an additional chemotaxonomic characteristic of sporeforming bacteria of the genera Bacillus and Clostridium | Bacillus thuringiensis | ? | - |
? | |
additional information | ability to cleave N-benzoyl-D-Arg-4-nitroanilide, i.e. D-BAPA is suggested as an additional chemotaxonomic characteristic of sporeforming bacteria of the genera Bacillus and Clostridium | Lysinibacillus sphaericus 2362 | ? | - |
? | |
additional information | ability to cleave N-benzoyl-D-Arg-4-nitroanilide, i.e. D-BAPA is suggested as an additional chemotaxonomic characteristic of sporeforming bacteria of the genera Bacillus and Clostridium | Bacillus cereus T | ? | - |
? | |
N-benzoyl-D-Arg-4-nitroanilide + H2O | ability to cleave D-BAPA is suggested as an additional chemotaxonomic characteristic of sporeforming bacteria of the genera Bacillus and Clostridium | Bacillus subtilis | N-benzoyl-D-Arg + 4-nitroaniline | - |
? | |
N-benzoyl-D-Arg-4-nitroanilide + H2O | ability to cleave D-BAPA is suggested as an additional chemotaxonomic characteristic of sporeforming bacteria of the genera Bacillus and Clostridium | Lysinibacillus sphaericus | N-benzoyl-D-Arg + 4-nitroaniline | - |
? | |
N-benzoyl-D-Arg-4-nitroanilide + H2O | ability to cleave D-BAPA is suggested as an additional chemotaxonomic characteristic of sporeforming bacteria of the genera Bacillus and Clostridium | Bacillus cereus | N-benzoyl-D-Arg + 4-nitroaniline | - |
? | |
N-benzoyl-D-Arg-4-nitroanilide + H2O | ability to cleave D-BAPA is suggested as an additional chemotaxonomic characteristic of sporeforming bacteria of the genera Bacillus and Clostridium | Clostridium sp. | N-benzoyl-D-Arg + 4-nitroaniline | - |
? | |
N-benzoyl-D-Arg-4-nitroanilide + H2O | ability to cleave D-BAPA is suggested as an additional chemotaxonomic characteristic of sporeforming bacteria of the genera Bacillus and Clostridium | Bacillaceae | N-benzoyl-D-Arg + 4-nitroaniline | - |
? | |
N-benzoyl-D-Arg-4-nitroanilide + H2O | ability to cleave D-BAPA is suggested as an additional chemotaxonomic characteristic of sporeforming bacteria of the genera Bacillus and Clostridium | Bacillus thuringiensis | N-benzoyl-D-Arg + 4-nitroaniline | - |
? | |
N-benzoyl-D-Arg-4-nitroanilide + H2O | D-BAPA, stereospecific to the D-isomer, no activity with the L-isomer | Bacillus subtilis | N-benzoyl-D-Arg + 4-nitroaniline | - |
? | |
N-benzoyl-D-Arg-4-nitroanilide + H2O | D-BAPA, stereospecific to the D-isomer, no activity with the L-isomer | Lysinibacillus sphaericus | N-benzoyl-D-Arg + 4-nitroaniline | - |
? | |
N-benzoyl-D-Arg-4-nitroanilide + H2O | D-BAPA, stereospecific to the D-isomer, no activity with the L-isomer | Bacillus cereus | N-benzoyl-D-Arg + 4-nitroaniline | - |
? | |
N-benzoyl-D-Arg-4-nitroanilide + H2O | D-BAPA, stereospecific to the D-isomer, no activity with the L-isomer | Clostridium sp. | N-benzoyl-D-Arg + 4-nitroaniline | - |
? | |
N-benzoyl-D-Arg-4-nitroanilide + H2O | D-BAPA, stereospecific to the D-isomer, no activity with the L-isomer | Bacillaceae | N-benzoyl-D-Arg + 4-nitroaniline | - |
? | |
N-benzoyl-D-Arg-4-nitroanilide + H2O | D-BAPA, stereospecific to the D-isomer, no activity with the L-isomer | Bacillus thuringiensis | N-benzoyl-D-Arg + 4-nitroaniline | - |
? | |
N-benzoyl-D-Arg-4-nitroanilide + H2O | D-BAPA, stereospecific to the D-isomer, no activity with the L-isomer | marine aerobic sporeforming bacterium 6A10 | N-benzoyl-D-Arg + 4-nitroaniline | - |
? | |
N-benzoyl-D-Arg-4-nitroanilide + H2O | ability to cleave D-BAPA is suggested as an additional chemotaxonomic characteristic of sporeforming bacteria of the genera Bacillus and Clostridium | Lysinibacillus sphaericus 2362 | N-benzoyl-D-Arg + 4-nitroaniline | - |
? | |
N-benzoyl-D-Arg-4-nitroanilide + H2O | D-BAPA, stereospecific to the D-isomer, no activity with the L-isomer | Lysinibacillus sphaericus 2362 | N-benzoyl-D-Arg + 4-nitroaniline | - |
? | |
N-benzoyl-D-Arg-4-nitroanilide + H2O | ability to cleave D-BAPA is suggested as an additional chemotaxonomic characteristic of sporeforming bacteria of the genera Bacillus and Clostridium | Bacillus cereus T | N-benzoyl-D-Arg + 4-nitroaniline | - |
? | |
N-benzoyl-D-Arg-4-nitroanilide + H2O | D-BAPA, stereospecific to the D-isomer, no activity with the L-isomer | Bacillus cereus T | N-benzoyl-D-Arg + 4-nitroaniline | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Bacillus subtilis |
45 | - |
- |
Bacillus cereus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
strain 168, Ca2+ and Mg2+ ions decrease thermostability | Bacillus subtilis |
60 | - |
strain 168, 2 min, 40% loss of activity in 0.05 M Tris buffer, pH 7.8, 60% loss of activity with 5 mM MgCl2, 80% loss of activity with 5 mM CaCl2 | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Bacillus subtilis |
7.8 | - |
assay at | Lysinibacillus sphaericus |
7.8 | - |
assay at | Bacillus cereus |
7.8 | - |
assay at | Clostridium sp. |
7.8 | - |
assay at | Bacillaceae |
7.8 | - |
assay at | Bacillus thuringiensis |
7.8 | - |
assay at | marine aerobic sporeforming bacterium 6A10 |