Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.1.71 extracted from

  • Martens, M.; Cottenie-Ruysschaert, M.; Hanselaer, R.; De Cooman, L.; Vande Casteele, K.; Van Sumere, C.F.
    N-Feruloylglycine amidohydrolase from barley seeds and isolated barley embryos (1988), Phytochemistry, 27, 2457-2463.
No PubMed abstract available
Search for more literature for 3.5.1.71

Activating Compound

Activating Compound Comment Organism Structure
(NH4)2SO4 0.4 M, enhances activity of unpurified enzyme by 55%, but no effect with purified enzyme Hordeum vulgare
K2SO4 0.4 M, enhances activity of unpurified enzyme by 36% Hordeum vulgare

General Stability

General Stability Organism
ammonium sulfate, 0.4 M, stabilizes during fractionation Hordeum vulgare
glycerol, 10%, stabilizes Hordeum vulgare

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information
-
 Hordeum vulgare
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-feruloylglycine + H2O Hordeum vulgare best substrate ferulate + glycine
-
 ?
N-feruloylglycine + H2O Hordeum vulgare enzyme increases in activity during the first 14 h, maximum activity, of germination: enzyme plays a role during germination ferulate + glycine
-
 ?
N-feruloylglycine + H2O Hordeum vulgare may be responsible for the hydrolysis of N-feruloylglycyl-moieties of certain reserve proteins, because such N-feruloylglycine-containing proteins occur in barley seeds ferulate + glycine
-
 ?

Organism

Organism UniProt Comment Textmining
Hordeum vulgare
-
 barley
-
Hordeum vulgare
-
 cv Zephyr and cv Gitane
-
Lactuca sativa
-
 lettuce, very low activity
-
Pisum sativum
-
 pea, very low activity
-
Secale cereale
-
 rye, low activity
-
Spinacia oleracea
-
 spinach, very low activity
-
Trifolium sp.
-
 clover, very low activity
-

Purification (Commentary)

Purification (Comment) Organism
from germinated and excised embryos Hordeum vulgare

Source Tissue

Source Tissue Comment Organism Textmining
aleurone layer
-
 Hordeum vulgare
-
embryo
-
 Pisum sativum
-
embryo
-
 Secale cereale
-
embryo highest activity in germinated barley embryos Hordeum vulgare
-
leaf
-
 Spinacia oleracea
-
leaf
-
 Lactuca sativa
-
leaf
-
 Trifolium sp.
-
additional information tissue distribution Hordeum vulgare
-
additional information tissue distribution Spinacia oleracea
-
additional information tissue distribution Pisum sativum
-
additional information tissue distribution Lactuca sativa
-
additional information tissue distribution Secale cereale
-
additional information tissue distribution Trifolium sp.
-
additional information no activity in endosperm, shoots and roots Hordeum vulgare
-
seed
-
 Hordeum vulgare
-
stem
-
 Trifolium sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Spinacia oleracea
additional information
-
-
 Pisum sativum
additional information
-
-
 Lactuca sativa
additional information
-
-
 Secale cereale
additional information
-
-
 Trifolium sp.
additional information
-
 highest activity in cv Gitane, almost twice the activity of cv Zephyr Hordeum vulgare
0.022
-
-
 Hordeum vulgare

Storage Stability

Storage Stability Organism
-28°C, stable for several months without serious loss of activity Hordeum vulgare

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with N-acetylglycine Hordeum vulgare ?
-
 ?
N-4-hydroxybenzoylglycine + H2O poorly hydrolyzed Hordeum vulgare 4-hydroxybenzoate + glycine
-
 ?
N-feruloylglycine + H2O
-
 Spinacia oleracea ferulate + glycine
-
 ?
N-feruloylglycine + H2O
-
 Pisum sativum ferulate + glycine
-
 ?
N-feruloylglycine + H2O
-
 Lactuca sativa ferulate + glycine
-
 ?
N-feruloylglycine + H2O
-
 Secale cereale ferulate + glycine
-
 ?
N-feruloylglycine + H2O
-
 Trifolium sp. ferulate + glycine
-
 ?
N-feruloylglycine + H2O best substrate Hordeum vulgare ferulate + glycine
-
 ?
N-feruloylglycine + H2O enzyme increases in activity during the first 14 h, maximum activity, of germination: enzyme plays a role during germination Hordeum vulgare ferulate + glycine
-
 ?
N-feruloylglycine + H2O may be responsible for the hydrolysis of N-feruloylglycyl-moieties of certain reserve proteins, because such N-feruloylglycine-containing proteins occur in barley seeds Hordeum vulgare ferulate + glycine
-
 ?

Synonyms

Synonyms Comment Organism
N-FGAH
-
 Hordeum vulgare

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Hordeum vulgare
32
-
-
 Hordeum vulgare

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
 stable up to, unstable above Hordeum vulgare

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Hordeum vulgare

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
 unstable below Hordeum vulgare
7.5 10 very stable between Hordeum vulgare