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Literature summary for 3.5.1.68 extracted from
- Purification and properties of formylglutamate amidohydrolase from Pseudomonas putida (1987), J. Bacteriol., 169, 4696-4702.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| histidine | induction and activation | Pseudomonas putida |  |
| N-formyl-L-glutamate | induction and activation | Pseudomonas putida | |
| Urocanate | induction and activation | Pseudomonas putida | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| hutG , formylglutamate amidohydrolase gene, subcloned on a multicopy plasmid in Escherichia coli | Pseudomonas putida |
| hutG, formylglutamate amidohydrolase gene, expressed in Escherichia coli RDP145 | Pseudomonas putida |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | slight inhibition above 0.1 mM, activation below 0.1 mM | Pseudomonas putida | |
| N-acetyl-L-glutamate | kinetics, competitive inhibition, Ki: 6 mM | Pseudomonas putida | |
| N-acetyl-L-glutamine | slight inhibition | Pseudomonas putida | |
| N-formyl-L-aspartate | kinetics, competitive inhibition, Ki: 9 mM | Pseudomonas putida | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 14 | - |
N-formyl-L-glutamate | - |
Pseudomonas putida | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | present in soluble fractions | Pseudomonas putida | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | no activation | Pseudomonas putida | |
| Co2+ | activation, most effective, 0.8 mM: 4fold activation | Pseudomonas putida | |
| Divalent metal ions | activation | Pseudomonas putida | |
| Fe2+ | activation below 0.1 mM, slight inhibition above 0.1 mM | Pseudomonas putida | |
| Mg2+ | no activation | Pseudomonas putida | |
| Mn2+ | no activation | Pseudomonas putida | |
| additional information | no activation by Cd2+ | Pseudomonas putida | |
| additional information | no activation by Ni2+, Zn2+ | Pseudomonas putida |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
1 * 50000, SDS-PAGE | Pseudomonas putida |
| 52000 | - |
gel filtration | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-formyl-L-glutamate + H2O | Pseudomonas putida | terminal reaction in the five-step pathway for histidine utilization | formate + L-glutamate | - |
? | |
| N-formyl-L-glutamate + H2O | Pseudomonas spp. | terminal reaction in the five-step pathway for histidine utilization | formate + L-glutamate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
wild-type strain PRS1, ATCC 12633 and several mutants | - |
| Pseudomonas spp. | - |
present in all species of Pseudomonas studied | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from recombinant Escherichia coli strain RDP145, pLH4 | Pseudomonas putida |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| N-formyl-L-glutamate + H2O = formate + L-glutamate | regulation, induction and catabolite repression of formylglutamate amidohydrolase | Pseudomonas putida |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Pseudomonas putida |
| 100 | - |
in the presence of 5 mM substrate, N-formyl-L-glutamate, and 0.8 mM CoCl2, 30°C | Pseudomonas putida |
| 380 | - |
calculated specific activity, with saturating substrate | Pseudomonas putida |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | substrate specificity | Pseudomonas putida | ? | - |
? | |
| additional information | little or no activity with N-acetyl-L-glutamate | Pseudomonas putida | ? | - |
? | |
| N-formyl-L-glutamate + H2O | terminal reaction in the five-step pathway for histidine utilization | Pseudomonas putida | formate + L-glutamate | - |
? | |
| N-formyl-L-glutamate + H2O | terminal reaction in the five-step pathway for histidine utilization | Pseudomonas spp. | formate + L-glutamate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 50000, SDS-PAGE | Pseudomonas putida |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Pseudomonas putida |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | maximally active at | Pseudomonas putida |
| 7.5 | - |
assay at | Pseudomonas putida |
| 7.5 | - |
100 mM potassium phosphate buffer | Pseudomonas putida |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.6 | 8.5 | more than 65% of maximal activity between pH 6.6 and 8.5 | Pseudomonas putida |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6 | - |
N-acetyl-L-glutamate | kinetics, competitive inhibition | Pseudomonas putida | |
| 9 | - |
N-formyl-L-aspartate | kinetics, competitive inhibition | Pseudomonas putida | |
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