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Literature summary for 3.5.1.68 extracted from

  • Asakura, M; Nagahashi, Y.; Hamada, M.; Kawai, M.; Kadobayashi, K.; Narahara, M.; Nakagawa, S.; Kawai, Y.; Hama, T.; Miyake, M.
    Purification and properties of beta-citryl-L-glutamate-hydrolysing enzyme from rat testis particulate (1995), Biochim. Biophys. Acta, 1250, 35-42.
    View publication on PubMed
Search for more literature for 3.5.1.68

Activating Compound

Activating Compound Comment Organism Structure
Lubrol Px enzyme solubilized, activation Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
AMP no or slight inhibition Rattus norvegicus
ATP 0.1 mM: 78% inhibition, 1 mM: 97% inhibition; strong competitive inhibition Rattus norvegicus
citrate 0.1 mM: 77% inhibition, 1 mM: 98% inhibition Rattus norvegicus
EDTA inhibition completely reversed by Mn2+, activity 30% restored by Ca2+ and 13% restored by Mg2+ Rattus norvegicus
EGTA
-
 Rattus norvegicus
glutamate 0.1 mM: 34% inhibition, 1 mM: 81% inhibition Rattus norvegicus
GMP no or slight inhibition Rattus norvegicus
GTP 0.1 mM: 90% inhibition, 1 mM: 99% inhibition; strong competitive inhibition Rattus norvegicus
L-Quisqualate 0.1 mM: 80% inhibition, 1 mM: 96% inhibition; non-competitive potent inhibitor Rattus norvegicus
additional information no or very slight inhibition by kinate, N-methyl-D-aspartate Rattus norvegicus
nucleotides strong inhibition Rattus norvegicus
phosphate
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0025
-
 beta-Citryl-L-glutamate
-
 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane localized in particulate fractions Rattus norvegicus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required for full activity, can replace Mn2+ to some extent Rattus norvegicus
Divalent metal ions required Rattus norvegicus
Fe2+ no activation Rattus norvegicus
Mg2+ required for full activity, can replace Mn2+ to some extent Rattus norvegicus
Mn2+ required for full activity Rattus norvegicus
Mn2+ maximal activity at 0.5-1 mM Rattus norvegicus
additional information enzyme is a metalloenzyme Rattus norvegicus
additional information no activation by Ni2+, Zn2+ Rattus norvegicus
additional information no activation by Cu2+ Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
350000
-
 PAGE, after digestion with N-glycosidase F Rattus norvegicus
380000
-
 gel filtration Rattus norvegicus
420000
-
 PAGE, before digestion with N-glycosidase F Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 Wistar
-
Rattus norvegicus Wistar
-
 Wistar
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
solubilized by 0.5% Lubrol PX, most effective detergent Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
testis
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0192
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-citryl-L-glutamate + H2O deacylation of beta-citryl-L-glutamate Rattus norvegicus citrate + L-glutamate
-
 ir
beta-citryl-L-glutamate + H2O best substrate, high substrate specificity for beta-citryl-L-glutamate Rattus norvegicus citrate + L-glutamate
-
 ir
beta-citryl-L-glutamine + H2O at about 1/30 of that for beta-citryl-L-glutamate Rattus norvegicus citrate + L-glutamine
-
 ir
additional information substrate specificity Rattus norvegicus ?
-
 ?
additional information little or no activity with N-formyl-L-aspartate, N-formyl-L-glutamine, N-acetyl-L-glutamate, alpha-citryl-L-glutamate, alpha- and beta-citryl-alpha-L-glutamylglutamate Rattus norvegicus ?
-
 ?
additional information no activity with N-acetyl-alpha-aspartylglutamate Rattus norvegicus ?
-
 ?
additional information little or no activity with N-acetyl-L-glutamate Rattus norvegicus ?
-
 ?
additional information substrate specificity Rattus norvegicus Wistar ?
-
 ?
additional information little or no activity with N-formyl-L-aspartate, N-formyl-L-glutamine, N-acetyl-L-glutamate, alpha-citryl-L-glutamate, alpha- and beta-citryl-alpha-L-glutamylglutamate Rattus norvegicus Wistar ?
-
 ?
additional information no activity with N-acetyl-alpha-aspartylglutamate Rattus norvegicus Wistar ?
-
 ?
additional information little or no activity with N-acetyl-L-glutamate Rattus norvegicus Wistar ?
-
 ?
N-acetyl-L-aspartate + H2O poor substrate Rattus norvegicus acetate + L-aspartate
-
 ?
N-formyl-L-glutamate + H2O poor substrate Rattus norvegicus formate + L-glutamate
-
 ?
N-formyl-L-glutamate + H2O poor substrate Rattus norvegicus Wistar formate + L-glutamate
-
 ?

Subunits

Subunits Comment Organism
multimer composed of 90000, 100000, 115000 and 130000 Da subunits, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 pI of about pH 4.3 Rattus norvegicus
6.5 7 in the presence of Mn2+ Rattus norvegicus