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Literature summary for 3.5.1.61 extracted from

  • Negi, V.S.; Bingham, J.P.; Li, Q.X.; Borthakur, D.
    A carbon-nitrogen lyase from Leucaena leucocephala catalyzes the first step of mimosine degradation (2014), Plant Physiol., 164, 922-934.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.61

Cloned(Commentary)

Cloned (Comment) Organism
gene seq3, DNA and amino acid sequence determination and analysis, codon optimization and recombinant expression in Escherichia coli Leucaena leucocephala

Inhibitors

Inhibitors Comment Organism Structure
hydroxylamine complete inhibition at 0.05 mM, reversible by 100 nM pyridoxal 5'-phosphate Leucaena leucocephala
additional information no competitive inhibition by L-tyrosine, L-phenylalanine, and L-tryptophan Leucaena leucocephala

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast the enzyme has a chloroplast transit peptide Leucaena leucocephala 9507
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 x * 45000 recombinant enzyme, SDS-PAGE Leucaena leucocephala

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-2-amino-3-(3-hydroxy-4-oxo-4H-pyridin-1-yl)propanoate + H2O Leucaena leucocephala
-
 3-hydroxy-4H-pyrid-4-one + pyruvate + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Leucaena leucocephala A8CEI3 gene seq3
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli Leucaena leucocephala

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Leucaena leucocephala
-
seedling
-
 Leucaena leucocephala
-
shoot
-
 Leucaena leucocephala
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-2-amino-3-(3-hydroxy-4-oxo-4H-pyridin-1-yl)propanoate + H2O
-
 Leucaena leucocephala 3-hydroxy-4H-pyrid-4-one + pyruvate + NH3
-
 ?
(S)-2-amino-3-(3-hydroxy-4-oxo-4H-pyridin-1-yl)propanoate + H2O i.e. mimosine, a toxic nonprotein aromatic amino acid. Dependence of the enzyme on pyridoxal 5'-phosphate and the production of 3-hydroxy-4H-pyrid-4-one with the release of ammonia indicate that the enzyme is a carbon-nitrogen lyase Leucaena leucocephala 3-hydroxy-4H-pyrid-4-one + pyruvate + NH3 product identification by by electrospray ionization-tandem mass spectrometry ?

Subunits

Subunits Comment Organism
? x * 45000 recombinant enzyme, SDS-PAGE Leucaena leucocephala

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Leucaena leucocephala

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
22 45 enzyme catalytic activity sharply decreases at 45°C or higher and below 22°C Leucaena leucocephala

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 purified recombinant enzyme, pH 8.5, 37°C, around 80% activity remaining, fairly stable up to 40°C Leucaena leucocephala
50
-
 purified recombinant enzyme, pH 8.5, 37°C, the enzyme activity shows a sharp reduction Leucaena leucocephala

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Leucaena leucocephala

pH Range

pH Minimum pH Maximum Comment Organism
7 12 high catalytic activities at pH 7.5-10.0, inactive below pH 7.0 Leucaena leucocephala

Cofactor

Cofactor Comment Organism Structure
additional information no requirement for an 2oxo acid Leucaena leucocephala
pyridoxal 5'-phosphate dependent on, enzyme-bound Leucaena leucocephala

General Information

General Information Comment Organism
metabolism the carbon-nitrogen lyase catalyzes the first step of mimosine degradation. Mimosine is a toxic nonprotein aromatic amino acid Leucaena leucocephala