Activating Compound | Comment | Organism | Structure |
---|---|---|---|
N-Carbamoyl-beta-alanine | causes association to more active higher molecular mass species | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli Rosetta (DE3)pLysS cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
T299C is crystallized by the hanging-drop vapor diffusion method at 20°C. The T299C mutant enzyme crystallizes in space group C2221 with one polypeptide chain per asymmetric unit. The structure is determined to 2.08 A by molecular replacement | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C233A | inactive mutant enzyme | Homo sapiens |
E137K | inactive mutant enzyme | Homo sapiens |
K132L | inactive mutant enzyme, the mutant enzyme is exclusively dimeric | Homo sapiens |
L13S | inactive mutant enzyme, the mutation is identified in beta-ureidopropionase-deficient patients | Homo sapiens |
additional information | disruption of dimer-dimer interfaces by site-directed mutagenesis generated dimeric, inactive enzyme variants | Homo sapiens |
R130D | inactive mutant enzyme | Homo sapiens |
R130D/S208R | inactive mutant enzyme, the mutant enzyme is exclusively dimeric | Homo sapiens |
R130I | inactive mutant enzyme, a significant monomer proportion is detected | Homo sapiens |
S208A | inactive mutant enzyme, a significant monomer proportion is detected | Homo sapiens |
S208C | inactive mutant enzyme, exclusively exists as dimer | Homo sapiens |
S208R | inactive mutant enzyme, the mutant enzyme is exclusively dimeric | Homo sapiens |
T299C | inactive mutant enzyme, exclusively exists as dimer | Homo sapiens |
T359M | inactive mutant enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-ureidobutyrate | - |
Homo sapiens | |
5-aminolevulinic acid | - |
Homo sapiens | |
beta-Alanine | causes dissociation to inactive dimers, competitive inhibition | Homo sapiens | |
beta-aminoisobutyrate | - |
Homo sapiens | |
gamma-aminobutyrate | - |
Homo sapiens | |
iodoacetamide | 30 min preincubation with 50 mM iodoacetamide renders the enzyme completely inactive, probably due to covalent modification of the active-site cysteine (C233) | Homo sapiens | |
additional information | preincubation of the enzyme with 1 mM EDTA has no influence on activity | Homo sapiens | |
Zn2+ | 5.5% residual activity is measured after preincubation with 50 mM ZnCl2 | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.019 | - |
3-ureidopropanoate | pH 7.4, 37°C | Homo sapiens | |
0.048 | - |
3-ureidopropanoate | pH 6.5, 37°C | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-ureidopropanoate + H2O | Homo sapiens | - |
beta-alanine + CO2 + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9UBR1 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-ureidopropanoate + H2O | - |
Homo sapiens | beta-alanine + CO2 + NH3 | - |
? | |
3-ureidopropanoate + H2O | 3-ureidopropanoate i.e. N-carbamoyl-beta-alanine | Homo sapiens | beta-alanine + CO2 + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | homodimer at pH 9, the enzyme increasingly associates to form octamers and larger oligomers with decreasing pH. Native PAGE analysis of wild-type enzyme indicates coexistence in at least five different oligomeric states, of which the smallest is most probably the dimer | Homo sapiens |
oligomer | homodimer at pH 9, the enzyme increasingly associates to form octamers and larger oligomers with decreasing pH. Native PAGE analysis of wild-type enzyme indicates coexistence in at least five different oligomeric states, of which the smallest is most probably the dimer | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
betaUP | - |
Homo sapiens |
UPB1 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.31 | - |
3-ureidopropanoate | pH 7.4, 37°C | Homo sapiens | |
0.47 | - |
3-ureidopropanoate | pH 6.5, 37°C | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0135 | - |
beta-Alanine | pH 6.5, 37°C | Homo sapiens | |
0.047 | - |
5-aminolevulinic acid | pH 6.5, 37°C | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme catalyzes the third step of the reductive pyrimidine catabolic pathway responsible for breakdown of uracil-, thymine- and pyrimidine-based antimetabolites such as 5-fluorouracil | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.8 | - |
3-ureidopropanoate | pH 6.5, 37°C | Homo sapiens | |
15.4 | - |
3-ureidopropanoate | pH 7.4, 37°C | Homo sapiens |