Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.1.6 extracted from

  • Maurer, D.; Lohkamp, B.; Krumpel, M.; Widersten, M.; Dobritzsch, D.
    Crystal structure and pH-dependent allosteric regulation of human beta-ureidopropionase, an enzyme involved in anticancer drug metabolism (2018), Biochem. J., 475, 2395-2416 .
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
N-Carbamoyl-beta-alanine causes association to more active higher molecular mass species Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Rosetta (DE3)pLysS cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
T299C is crystallized by the hanging-drop vapor diffusion method at 20°C. The T299C mutant enzyme crystallizes in space group C2221 with one polypeptide chain per asymmetric unit. The structure is determined to 2.08 A by molecular replacement Homo sapiens

Protein Variants

Protein Variants Comment Organism
C233A inactive mutant enzyme Homo sapiens
E137K inactive mutant enzyme Homo sapiens
K132L inactive mutant enzyme, the mutant enzyme is exclusively dimeric Homo sapiens
L13S inactive mutant enzyme, the mutation is identified in beta-ureidopropionase-deficient patients Homo sapiens
additional information disruption of dimer-dimer interfaces by site-directed mutagenesis generated dimeric, inactive enzyme variants Homo sapiens
R130D inactive mutant enzyme Homo sapiens
R130D/S208R inactive mutant enzyme, the mutant enzyme is exclusively dimeric Homo sapiens
R130I inactive mutant enzyme, a significant monomer proportion is detected Homo sapiens
S208A inactive mutant enzyme, a significant monomer proportion is detected Homo sapiens
S208C inactive mutant enzyme, exclusively exists as dimer Homo sapiens
S208R inactive mutant enzyme, the mutant enzyme is exclusively dimeric Homo sapiens
T299C inactive mutant enzyme, exclusively exists as dimer Homo sapiens
T359M inactive mutant enzyme Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
4-ureidobutyrate
-
Homo sapiens
5-aminolevulinic acid
-
Homo sapiens
beta-Alanine causes dissociation to inactive dimers, competitive inhibition Homo sapiens
beta-aminoisobutyrate
-
Homo sapiens
gamma-aminobutyrate
-
Homo sapiens
iodoacetamide 30 min preincubation with 50 mM iodoacetamide renders the enzyme completely inactive, probably due to covalent modification of the active-site cysteine (C233) Homo sapiens
additional information preincubation of the enzyme with 1 mM EDTA has no influence on activity Homo sapiens
Zn2+ 5.5% residual activity is measured after preincubation with 50 mM ZnCl2 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.019
-
3-ureidopropanoate pH 7.4, 37°C Homo sapiens
0.048
-
3-ureidopropanoate pH 6.5, 37°C Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-ureidopropanoate + H2O Homo sapiens
-
beta-alanine + CO2 + NH3
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9UBR1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-ureidopropanoate + H2O
-
Homo sapiens beta-alanine + CO2 + NH3
-
?
3-ureidopropanoate + H2O 3-ureidopropanoate i.e. N-carbamoyl-beta-alanine Homo sapiens beta-alanine + CO2 + NH3
-
?

Subunits

Subunits Comment Organism
homodimer homodimer at pH 9, the enzyme increasingly associates to form octamers and larger oligomers with decreasing pH. Native PAGE analysis of wild-type enzyme indicates coexistence in at least five different oligomeric states, of which the smallest is most probably the dimer Homo sapiens
oligomer homodimer at pH 9, the enzyme increasingly associates to form octamers and larger oligomers with decreasing pH. Native PAGE analysis of wild-type enzyme indicates coexistence in at least five different oligomeric states, of which the smallest is most probably the dimer Homo sapiens

Synonyms

Synonyms Comment Organism
betaUP
-
Homo sapiens
UPB1
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.31
-
3-ureidopropanoate pH 7.4, 37°C Homo sapiens
0.47
-
3-ureidopropanoate pH 6.5, 37°C Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0135
-
beta-Alanine pH 6.5, 37°C Homo sapiens
0.047
-
5-aminolevulinic acid pH 6.5, 37°C Homo sapiens

General Information

General Information Comment Organism
metabolism the enzyme catalyzes the third step of the reductive pyrimidine catabolic pathway responsible for breakdown of uracil-, thymine- and pyrimidine-based antimetabolites such as 5-fluorouracil Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
9.8
-
3-ureidopropanoate pH 6.5, 37°C Homo sapiens
15.4
-
3-ureidopropanoate pH 7.4, 37°C Homo sapiens