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Literature summary for 3.5.1.6 extracted from

  • Traut, T.W.; Loechel, S.
    Pyrimidine catabolism: individual characterization of the three sequential enzymes with a new assay (1984), Biochemistry, 23, 2533-2539.
    View publication on PubMed
Search for more literature for 3.5.1.6

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0235
-
 N-Carbamoyl-beta-alanine
-
 Rattus rattus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Rattus rattus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
234000
-
 gel filtration Rattus rattus
340000
-
 gel filtration Rattus rattus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-carbamoyl-beta-alanine + H2O Mus musculus
-
 ?
-
 ?
N-carbamoyl-beta-alanine + H2O Escherichia coli
-
 ?
-
 ?
N-carbamoyl-beta-alanine + H2O Euglena gracilis
-
 ?
-
 ?
N-carbamoyl-beta-alanine + H2O Rattus rattus
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Euglena gracilis
-
 plant-like unicellular flagellate
-
Mus musculus
-
 mouse
-
Rattus rattus
-
 rat
-

Source Tissue

Source Tissue Comment Organism Textmining
hepatoma cell
-
 Rattus rattus
-
liver
-
 Mus musculus
-
liver
-
 Rattus rattus
-

Storage Stability

Storage Stability Organism
4°C, 50 mM Tris buffer with 10% glycerol + 10 mM 2-mercaptoethanol or 2 mM dithiothreitol enzyme loses less than 20% activity when maintained for 3 days Rattus rattus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-carbamoyl-beta-alanine + H2O L enantiomer specific hydrolysis Mus musculus beta-alanine + CO2 + NH3
-
 ir
N-carbamoyl-beta-alanine + H2O L enantiomer specific hydrolysis Escherichia coli beta-alanine + CO2 + NH3
-
 ir
N-carbamoyl-beta-alanine + H2O L enantiomer specific hydrolysis Euglena gracilis beta-alanine + CO2 + NH3
-
 ir
N-carbamoyl-beta-alanine + H2O L enantiomer specific hydrolysis Rattus rattus beta-alanine + CO2 + NH3
-
 ir
N-carbamoyl-beta-alanine + H2O
-
 Mus musculus ?
-
 ?
N-carbamoyl-beta-alanine + H2O
-
 Escherichia coli ?
-
 ?
N-carbamoyl-beta-alanine + H2O
-
 Euglena gracilis ?
-
 ?
N-carbamoyl-beta-alanine + H2O
-
 Rattus rattus ?
-
 ?