Any feedback?
Literature summary for 3.5.1.6 extracted from
- Purification and properties of beta-ureidopropionase from the rat liver (1987), Eur. J. Biochem., 169, 21-26.
General Stability
| General Stability | Organism |
|---|---|
| stored in 50% glycerol without gross denaturation | Rattus rattus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0235 | 0.5 | N-Carbamoyl-beta-alanine | pH dependent | Rattus rattus |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Rattus rattus | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | - |
Rattus rattus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54000 | - |
6 * 54000, hexamer is composed of 3 dimers of 115000, SDS-PAGE | Rattus rattus |
| 323000 | - |
sucrose density gradient centrifugation | Rattus rattus |
| 327000 | - |
gel filtration | Rattus rattus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-carbamoyl-beta-alanine + H2O | Rattus rattus | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus rattus | - |
rat | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purified 1000fold | Rattus rattus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Rattus rattus | - |
| gastrocnemius | - |
Rattus rattus | - |
| heart | - |
Rattus rattus | - |
| kidney | - |
Rattus rattus | - |
| large intestine | - |
Rattus rattus | - |
| liver | - |
Rattus rattus | - |
| lung | - |
Rattus rattus | - |
| pancreas | - |
Rattus rattus | - |
| small intestine | - |
Rattus rattus | - |
| spleen | - |
Rattus rattus | - |
| stomach | - |
Rattus rattus | - |
| testis | - |
Rattus rattus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.59 | - |
- |
Rattus rattus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -25°C 50% decrease in specific activity within a few days 4°C, 50% glycerol solution 20% decrease in activity at 1 month, 50% decrease at 2 months of storage | Rattus rattus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-carbamoyl-beta-alanine + H2O | L enantiomer specific hydrolysis | Rattus rattus | beta-alanine + CO2 + NH3 | - |
ir | |
| N-carbamoyl-beta-alanine + H2O | - |
Rattus rattus | ? | - |
? | |
| N-carbamoyl-DL-alanine + H2O | poor substrate, relative enzyme activity 4% | Rattus rattus | DL-alanine + CO2 + NH3 | - |
? | |
| N-carbamoyl-DL-beta-aminoisobutyrate + H2O | - |
Rattus rattus | DL-beta-aminoisobutyrate + CO2 + NH3 | - |
? | |
| N-carbamoylglycine + H2O | poor substrate, relative enzyme activity 1% | Rattus rattus | glycine + CO2 + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 54000, hexamer is composed of 3 dimers of 115000, SDS-PAGE | Rattus rattus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | - |
Rattus rattus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.3 | 8.3 | - |
Rattus rattus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
