Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DTT | - |
Enterococcus faecalis | |
EDTA | - |
Enterococcus faecalis | |
iodoacetamide | weak | Enterococcus faecalis | |
iodoacetate | weak | Enterococcus faecalis | |
N-benzyloxycarbonyl-L-Phe chloromethyl ketone | weak | Enterococcus faecalis | |
o-phenanthroline | weak | Enterococcus faecalis | |
p-chloromercuribenzoate | complete loss of activity, inhibition reversible by 2-mercaptoethanol | Enterococcus faecalis | |
p-tosyl-L-Lys chloromethyl ketone | weak | Enterococcus faecalis | |
p-tosyl-L-Phe chloromethyl ketone | weak | Enterococcus faecalis | |
PMSF | weak | Enterococcus faecalis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | very slight activation | Enterococcus faecalis | |
Ca2+ | very slight activation | Enterococcus faecalis | |
Cd2+ | very slight activation | Enterococcus faecalis | |
Co2+ | - |
Enterococcus faecalis | |
Fe2+ | very slight activation | Enterococcus faecalis | |
Mn2+ | - |
Enterococcus faecalis | |
Pb2+ | very slight activation | Enterococcus faecalis | |
Zn2+ | - |
Enterococcus faecalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
220000 | - |
gel filtration | Enterococcus faecalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus faecalis | - |
- |
- |
Enterococcus faecalis R / ATCC 8043 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Enterococcus faecalis |
Subunits | Comment | Organism |
---|---|---|
octamer | octamer postulated, dimer or tetramer possible in case of aggregation of the native enzyme in gel filtration, SDS-PAGE | Enterococcus faecalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Enterococcus faecalis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
- |
stable for 60 days at pH 6.0, 80% of activity remaining after 3 months, 50% of activity remaining after 6 months | Enterococcus faecalis |
20 | - |
stable for 60 min below 20°C in 0.05 M phosphate buffer, pH 6.0 | Enterococcus faecalis |
25 | - |
stable for 16 h around pH 6.0 | Enterococcus faecalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Enterococcus faecalis |