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Literature summary for 3.5.1.57 extracted from

  • Iwayama, A.; Kimura, T.; Adachi, O.; Ameyama, M.
    Crystallization and characterization of a novel aminopeptidase from Trichosporon cutaneum (1983), Agric. Biol. Chem., 47, 2483-2493.
No PubMed abstract available
Search for more literature for 3.5.1.57

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Cutaneotrichosporon cutaneum

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline weak Cutaneotrichosporon cutaneum
2,2'-dipyridyl
-
 Cutaneotrichosporon cutaneum
monoiodoacetate weak Cutaneotrichosporon cutaneum
NEM
-
 Cutaneotrichosporon cutaneum
PCMB weak Cutaneotrichosporon cutaneum
PMSF weak Cutaneotrichosporon cutaneum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.6
-
 L-Tryptophanamide
-
 Cutaneotrichosporon cutaneum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Cutaneotrichosporon cutaneum 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ some activation Cutaneotrichosporon cutaneum
Mn2+ required for activity Cutaneotrichosporon cutaneum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
 4 * 68000, SDS-PAGE Cutaneotrichosporon cutaneum
270000
-
 gel filtration Cutaneotrichosporon cutaneum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-tryptophanamide + H2O Cutaneotrichosporon cutaneum
-
 L-tryptophan + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Cutaneotrichosporon cutaneum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cutaneotrichosporon cutaneum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1110
-
-
 Cutaneotrichosporon cutaneum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alaninamide + H2O 14% of the activity with L-tryptophanamide Cutaneotrichosporon cutaneum L-alanine + NH3
-
 ?
L-asparagine + H2O 22% of the activity with L-tryptophanamide Cutaneotrichosporon cutaneum L-aspartate + NH3
-
 ?
L-citrullin + H2O 22% of the activity with L-tryptophanamide Cutaneotrichosporon cutaneum ?
-
 ?
L-glutamine + H2O 18% of the activity with L-tryptophanamide Cutaneotrichosporon cutaneum L-glutamate + NH3
-
 ?
L-leucinamide + H2O 26% of the activity with L-tryptophanamide Cutaneotrichosporon cutaneum L-leucine + NH3
-
 ?
L-methioninamide + H2O 14% of the activity with L-tryptophanamide Cutaneotrichosporon cutaneum L-methionine + NH3
-
 ?
L-phenylalaninamide + H2O 47% of the activity with L-tryptophanamide Cutaneotrichosporon cutaneum L-phenylalanine + NH3
-
 ?
L-serinamide + H2O 7% of the activity with L-tryptophanamide Cutaneotrichosporon cutaneum L-serine + NH3
-
 ?
L-tryptophanamide + H2O
-
 Cutaneotrichosporon cutaneum L-tryptophan + NH3
-
 ?
L-tyrosinamide + H2O 22% of the activity with L-tryptophanamide Cutaneotrichosporon cutaneum L-tyrosine + NH3
-
 ?
L-valinamide + H2O 15% of the activity with L-tryptophanamide Cutaneotrichosporon cutaneum L-valine + NH3
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 68000, SDS-PAGE Cutaneotrichosporon cutaneum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 45 crystalline enzyme Cutaneotrichosporon cutaneum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 60 crystalline enzyme, 35°C: about 75% of activity maximum, 60°C: about 35% of activity maximum Cutaneotrichosporon cutaneum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 crystalline enzyme, 10 min, stable Cutaneotrichosporon cutaneum
60
-
 crystalline enzyme, rapid inactivation Cutaneotrichosporon cutaneum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9 9.5 crystalline enzyme Cutaneotrichosporon cutaneum

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.5 8.5 crystalline enzyme, pH 7.5, 20 h, room temperature Cutaneotrichosporon cutaneum