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Literature summary for 3.5.1.56 extracted from

  • Hasegawa, Y.; Matsuo, M.; Sigemoto, Y.; Sakai, T.; Tokuyama, T.
    Purification and characterisation of N,N-dimethylformamidase from Alcaligenes sp. KUFA-1 (1997), J. Ferment. Bioeng., 84, 543-547.
No PubMed abstract available
Search for more literature for 3.5.1.56

Inhibitors

Inhibitors Comment Organism Structure
2,2'-dipyridyl
-
 Alcaligenes sp.
8-hydroxyquinoline
-
 Alcaligenes sp.
EDTA
-
 Alcaligenes sp.
Mg2+ 64% inhibition, 1 mM Alcaligenes sp.
Mn2+ 50% inhibition, 1 mM Alcaligenes sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.28
-
 N,N-Dimethylformamide
-
 Alcaligenes sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16000
-
 alpha2beta2, 2 * 16000 + 2 * 74000 Alcaligenes sp.
74000
-
 alpha2beta2, 2 * 16000 + 2 * 74000 Alcaligenes sp.
180000
-
 gel filtration Alcaligenes sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N,N-dimethylformamide + H2O Alcaligenes sp.
-
 dimethylamine + formate
-
 ?
N,N-dimethylformamide + H2O Alcaligenes sp. KUFA-1
-
 dimethylamine + formate
-
 ?

Organism

Organism UniProt Comment Textmining
Alcaligenes sp.
-
-
-
Alcaligenes sp. KUFA-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Alcaligenes sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
26.25
-
-
 Alcaligenes sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N,N-diethylformamide + H2O 29% of the activity with N,N-dimethylformamide Alcaligenes sp. diethylamine + formate
-
 ?
N,N-diethylformamide + H2O 29% of the activity with N,N-dimethylformamide Alcaligenes sp. KUFA-1 diethylamine + formate
-
 ?
N,N-dimethylacetamide + H2O 31% of the activity with N,N-dimethylformamide Alcaligenes sp. dimethylamine + acetate
-
 ?
N,N-dimethylacetamide + H2O 31% of the activity with N,N-dimethylformamide Alcaligenes sp. KUFA-1 dimethylamine + acetate
-
 ?
N,N-dimethylformamide + H2O
-
 Alcaligenes sp. dimethylamine + formate
-
 ?
N,N-dimethylformamide + H2O
-
 Alcaligenes sp. KUFA-1 dimethylamine + formate
-
 ?

Subunits

Subunits Comment Organism
tetramer alpha2beta2, 2 * 16000 + 2 * 74000 Alcaligenes sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Alcaligenes sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 60
-
 Alcaligenes sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 30 min, stable Alcaligenes sp.
60
-
 30 min, complete loss of activity Alcaligenes sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.6
-
-
 Alcaligenes sp.

pH Range

pH Minimum pH Maximum Comment Organism
4 11
-
 Alcaligenes sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1
-
 Mn2+
-
 Alcaligenes sp.