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Literature summary for 3.5.1.55 extracted from

  • Fukuda, H.; Iwade, S.; Kimura, A.
    A new enzyme: long acyl aminoacylase from Pseudomonas diminuta (1982), J. Biochem., 91, 1731-1738.
    View publication on PubMed
Search for more literature for 3.5.1.55

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ slight inhibition Brevundimonas diminuta
Cu2+ slight inhibition Brevundimonas diminuta
Hg2+
-
 Brevundimonas diminuta

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.083
-
 N-myristoyl-L-glutamate
-
 Brevundimonas diminuta
0.14
-
 N-palmitoyl-L-glutamate
-
 Brevundimonas diminuta
0.17
-
 N-lauroyl-L-glutamate
-
 Brevundimonas diminuta
0.33
-
 N-stearoyl-L-glutamate
-
 Brevundimonas diminuta

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
300000
-
 enzyme I, gel filtration Brevundimonas diminuta

Organism

Organism UniProt Comment Textmining
Brevundimonas diminuta
-
-
-

Purification (Commentary)

Purification (Comment) Organism
enzyme I Brevundimonas diminuta

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2933
-
-
 Brevundimonas diminuta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information not: acetyl-L-glutamate Brevundimonas diminuta ?
-
 ?
additional information does not act on acyl derivatives of other amino acids Brevundimonas diminuta ?
-
 ?
N-lauroyl-L-aspartate + H2O
-
 Brevundimonas diminuta lauroate + L-aspartate
-
 ?
N-lauroyl-L-glutamate + H2O C12, 99% of the activity with pentadecanoyl-L-glutamate Brevundimonas diminuta lauroate + L-glutamate
-
 ?
N-myristoyl-L-glutamate + H2O C14, 97% of the activity with pentadecanoyl-L-glutamate Brevundimonas diminuta myristoate + L-glutamate
-
 ?
N-palmitoyl-L-glutamate + H2O C16, 60% of the activity with pentadecanoyl-L-glutamate Brevundimonas diminuta palmitate + L-glutamate
-
 ?
N-pentadecanoyl-L-glutamate + H2O best substrate Brevundimonas diminuta pentadecanoate + L-glutamate
-
 ?
N-stearoyl-L-glutamate + H2O 36% of the activity with pentadecanoyl-L-glutamate Brevundimonas diminuta stearate + L-glutamate
-
 ?
N-undecanoyl-L-glutamate + H2O 73% of the activity with pentadecanoyl-L-glutamate Brevundimonas diminuta undecanoate + L-glutamate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Brevundimonas diminuta

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 pH 7, 10 min, quite stable, enzyme form I Brevundimonas diminuta
70
-
 pH 7, 10 min, complete inactivation, enzyme form I Brevundimonas diminuta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 substrate N-lauroyl-L-glutamate, N-myristoyl-L-glutamate Brevundimonas diminuta
8.5
-
 substrate N-palmitoyl-L-glutamate Brevundimonas diminuta
9
-
 substrate N-tearoyl-L-glutamate Brevundimonas diminuta

pH Stability

pH Stability pH Stability Maximum Comment Organism
6
-
 4°C, 24 h, 60% loss of activity, enzyme form I Brevundimonas diminuta
7 11 4°C, 24 h, stable, enzyme form I Brevundimonas diminuta