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Literature summary for 3.5.1.54 extracted from

  • Cheng, G.; Shapir, N.; Sadowsky, M.J.; Wackett, L.P.
    Allophanate hydrolase, not urease, functions in bacterial cyanuric acid metabolism (2005), Appl. Environ. Microbiol., 71, 4437-4445.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
sequence analysis and comparison, and phylogenetic analysis Agrobacterium tumefaciens
sequence analysis and comparison, and phylogenetic analysis Ralstonia pickettii
sequence analysis and comparison, and phylogenetic analysis Herbaspirillum huttiense
sequence analysis and comparison, and phylogenetic analysis Enterobacter cloacae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
allophanate + H2O Agrobacterium tumefaciens the enzyme is involved in bacterial cyanuric acid metabolism NH3 + CO2
-
ir
allophanate + H2O Ralstonia pickettii the enzyme is involved in bacterial cyanuric acid metabolism NH3 + CO2
-
ir
allophanate + H2O Pseudomonas sp. the enzyme is involved in bacterial cyanuric acid metabolism NH3 + CO2
-
ir
allophanate + H2O Herbaspirillum huttiense the enzyme is involved in bacterial cyanuric acid metabolism NH3 + CO2
-
ir
allophanate + H2O Enterobacter cloacae the enzyme is involved in bacterial cyanuric acid metabolism NH3 + CO2
-
ir
allophanate + H2O Herbaspirillum huttiense NRRL B-12228 the enzyme is involved in bacterial cyanuric acid metabolism NH3 + CO2
-
ir
allophanate + H2O Agrobacterium tumefaciens J14a the enzyme is involved in bacterial cyanuric acid metabolism NH3 + CO2
-
ir
allophanate + H2O Ralstonia pickettii D the enzyme is involved in bacterial cyanuric acid metabolism NH3 + CO2
-
ir
allophanate + H2O Enterobacter cloacae 99 the enzyme is involved in bacterial cyanuric acid metabolism NH3 + CO2
-
ir

Organism

Organism UniProt Comment Textmining
Agrobacterium tumefaciens
-
gene atzF
-
Agrobacterium tumefaciens J14a
-
gene atzF
-
Enterobacter cloacae Q9ALV2
-
-
Enterobacter cloacae 99 Q9ALV2
-
-
Herbaspirillum huttiense
-
gene atzF
-
Herbaspirillum huttiense NRRL B-12228
-
gene atzF
-
Pseudomonas sp. Q936X2 gene atzF
-
Ralstonia pickettii
-
gene atzF
-
Ralstonia pickettii D
-
gene atzF
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
allophanate + H2O
-
Agrobacterium tumefaciens NH3 + CO2
-
ir
allophanate + H2O
-
Ralstonia pickettii NH3 + CO2
-
ir
allophanate + H2O
-
Pseudomonas sp. NH3 + CO2
-
ir
allophanate + H2O
-
Herbaspirillum huttiense NH3 + CO2
-
ir
allophanate + H2O
-
Enterobacter cloacae NH3 + CO2
-
ir
allophanate + H2O the enzyme is involved in bacterial cyanuric acid metabolism Agrobacterium tumefaciens NH3 + CO2
-
ir
allophanate + H2O the enzyme is involved in bacterial cyanuric acid metabolism Ralstonia pickettii NH3 + CO2
-
ir
allophanate + H2O the enzyme is involved in bacterial cyanuric acid metabolism Pseudomonas sp. NH3 + CO2
-
ir
allophanate + H2O the enzyme is involved in bacterial cyanuric acid metabolism Herbaspirillum huttiense NH3 + CO2
-
ir
allophanate + H2O the enzyme is involved in bacterial cyanuric acid metabolism Enterobacter cloacae NH3 + CO2
-
ir
allophanate + H2O
-
Herbaspirillum huttiense NRRL B-12228 NH3 + CO2
-
ir
allophanate + H2O the enzyme is involved in bacterial cyanuric acid metabolism Herbaspirillum huttiense NRRL B-12228 NH3 + CO2
-
ir
allophanate + H2O
-
Agrobacterium tumefaciens J14a NH3 + CO2
-
ir
allophanate + H2O the enzyme is involved in bacterial cyanuric acid metabolism Agrobacterium tumefaciens J14a NH3 + CO2
-
ir
allophanate + H2O
-
Ralstonia pickettii D NH3 + CO2
-
ir
allophanate + H2O the enzyme is involved in bacterial cyanuric acid metabolism Ralstonia pickettii D NH3 + CO2
-
ir
allophanate + H2O
-
Enterobacter cloacae 99 NH3 + CO2
-
ir
allophanate + H2O the enzyme is involved in bacterial cyanuric acid metabolism Enterobacter cloacae 99 NH3 + CO2
-
ir
additional information allophanate also shows nonenzymatic decomposition, half-life at pH 8.0 is 50 h Pseudomonas sp. ?
-
?

Synonyms

Synonyms Comment Organism
AtzF
-
Agrobacterium tumefaciens
AtzF
-
Ralstonia pickettii
AtzF
-
Pseudomonas sp.
AtzF
-
Herbaspirillum huttiense
AtzF
-
Enterobacter cloacae
TrzF
-
Enterobacter cloacae
urea amidolyase
-
Enterobacter cloacae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Pseudomonas sp.