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Literature summary for 3.5.1.54 extracted from

  • Maitz, G.S.; Haas, E.M.; Castric, P.A.
    Purification and properties of the allophanate hydrolase from Chlamydomonas reinhardii (1982), Biochim. Biophys. Acta, 714, 486-491.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoic acid)
-
Chlamydomonas reinhardtii
acetoacetic acid Ki 0.3 mM Chlamydomonas reinhardtii
malonic acid
-
Chlamydomonas reinhardtii
NEM allophanate protects Chlamydomonas reinhardtii
oxaloacetic acid
-
Chlamydomonas reinhardtii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.55
-
allophanate
-
Chlamydomonas reinhardtii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
gel filtration Chlamydomonas reinhardtii
110000
-
PAGE Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
allophanate + H2O Chlamydomonas reinhardtii 2nd step in the degradation of urea CO2 + NH3
-
ir

Organism

Organism UniProt Comment Textmining
Chlamydomonas reinhardtii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Chlamydomonas reinhardtii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
19.3
-
-
Chlamydomonas reinhardtii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
allophanate + H2O
-
Chlamydomonas reinhardtii CO2 + NH3
-
ir
allophanate + H2O 2nd step in the degradation of urea Chlamydomonas reinhardtii CO2 + NH3
-
ir

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Chlamydomonas reinhardtii

pH Range

pH Minimum pH Maximum Comment Organism
7 10 pH 7.0: about 65% of maximal activity, pH 10.0: about 30% of maximal activity Chlamydomonas reinhardtii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.3
-
acetoacetic acid
-
Chlamydomonas reinhardtii