Application | Comment | Organism |
---|---|---|
medicine | site targeted delivery of urease along with chemotherapeutic drugs can be a promising tool for anti-cancer applications. Urease shows its inhibitory effects on cancer cell lines through the generation of toxic ammonia, which in turn increases the pH of the surrounding medium. This increase in extracellular pH, enhances the cytotoxic effect of weak base chemotherapeutic drugs, doxorubicin (0.05 mM) and vinblastine (0.100 mM) in the presence of urease (5 U/ml) and urea (0-4 mM) significantly | Glutamicibacter creatinolyticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
16.21 | - |
Urea | pH and temperature not specified in the publication | Glutamicibacter creatinolyticus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Glutamicibacter creatinolyticus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
270000 | - |
non-denaturing PAGE | Glutamicibacter creatinolyticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
urea + H2O | Glutamicibacter creatinolyticus | - |
CO2 + 2 NH3 | - |
? | |
urea + H2O | Glutamicibacter creatinolyticus MTCC 5604 | - |
CO2 + 2 NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glutamicibacter creatinolyticus | - |
- |
- |
Glutamicibacter creatinolyticus MTCC 5604 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Glutamicibacter creatinolyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
urea + H2O | - |
Glutamicibacter creatinolyticus | CO2 + 2 NH3 | - |
? | |
urea + H2O | - |
Glutamicibacter creatinolyticus MTCC 5604 | CO2 + 2 NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | the purified urease shows three bands with molecular weight of 72600 Da, 11200 Da and 6100 Da, SDS PAGE | Glutamicibacter creatinolyticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Glutamicibacter creatinolyticus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 80 | 20°C: about 40% of maximal activity, 80°C: about 45% of maximal activity | Glutamicibacter creatinolyticus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 50 | 1 h pH 8.0, stable between 4°C and 50°C | Glutamicibacter creatinolyticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Glutamicibacter creatinolyticus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | pH 6.0: about 40% of maximal activity, pH 10.0: about 50% of maximal activity | Glutamicibacter creatinolyticus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | 1 h 30°C, stable within the pH range of 7.0-9.0 | Glutamicibacter creatinolyticus |