Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21(DE3) | Klebsiella aerogenes |
Protein Variants | Comment | Organism |
---|---|---|
C319A | mutation increases the urease resistance to both epigallocatechin and quercetin | Klebsiella aerogenes |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
epigallocatechin | strong time-dependent inactivation of urease that is not due to their oxygen sensitivity. Rather, the compound appears to inactivate urease by reacting with a specific Cys residue located on the flexible loop. Substitution of this cysteine by alanine in the C319A variant increases the urease resistance | Klebsiella aerogenes | |
additional information | caftaric acid, desmosine, kanamycin, and labetalol, each examined at concentrations up to 0.6 mM, show little to no inhibitory activity | Klebsiella aerogenes | |
quercetin | strong time-dependent inactivation of urease that is not due to their oxygen sensitivity. Rather, the compound appears to inactivate urease by reacting with a specific Cys residue located on the flexible loop. Substitution of this cysteine by alanine in the C319A variant increases the urease resistance | Klebsiella aerogenes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
urea + H2O | Klebsiella aerogenes | - |
CO2 + 2 NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella aerogenes | P18314 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Klebsiella aerogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
urea + H2O | - |
Klebsiella aerogenes | CO2 + 2 NH3 | - |
? |