Application | Comment | Organism |
---|---|---|
biotechnology | soybean (Glycine max) urease is immobilized on alginate and chitosan beads and shows improved stability. This could have a potential role in haemodialysis machines | Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.7 | - |
Urea | soluble urease, Vmax: 285 micromol/min/mg | Glycine max | |
3.92 | - |
Urea | chitosan-immobilized urease, Vmax: 265 micromol/min/mg | Glycine max | |
5.88 | - |
Urea | alginate-immobilized urease, Vmax: 282 micromol/min/mg | Glycine max |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
urea + H2O | - |
Glycine max | CO2 + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
urease | - |
Glycine max |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
soluble urease | Glycine max |
75 | - |
chitosan-immobilized urease | Glycine max |
80 | - |
alginate-immobilized urease | Glycine max |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
immobilized urease shows improved stability when stored at 4°C and t1/2 of urease is found to be 19 days, 80 days and 121 days, respectively for soluble, alginate and chitosan ureases | Glycine max |
75 | - |
immobilized urease shows improved stability at 75°C. Soluble urease t1/2: 12 min, chitosan-immobilized urease t1/2: 58 min, alginate-immobilized urease t1/2: 43 min | Glycine max |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.2 | - |
alginate-immobilized urease | Glycine max |
7 | - |
soluble urease | Glycine max |
7.9 | - |
chitosan-immobilized urease | Glycine max |