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Literature summary for 3.5.1.5 extracted from

  • Andrich, L.; Esti, M.; Moresi, M.
    Urea degradation kinetics in model wine solutions by acid urease immobilised onto chitosan-derivative beads of different sizes (2010), Enzyme Microb. Technol., 46, 397-405.
No PubMed abstract available

Application

Application Comment Organism
biotechnology in food industry immobilisation of acid urease on an inert carrier has the potential advantages of significant cost savings, improved stability or resistance to shear or inhibitory compound inactivation. Purified acid urease preparation is covalently immobilised onto biocompatible porous chitosan beads of different size. The kinetics of urea degradation in a model wine solution using this biocatalyst is of the pseudofirst order with respect to the urea concentration in the liquid bulk, the apparent pseudo-first order kinetic rate constant ranging from about two thirds to one fifth of that pertaining to free acid urease Limosilactobacillus fermentum

Organism

Organism UniProt Comment Textmining
Limosilactobacillus fermentum
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.647
-
20°C, pH 4 Limosilactobacillus fermentum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
urea + H2O
-
Limosilactobacillus fermentum CO2 + NH3
-
?

Synonyms

Synonyms Comment Organism
urease
-
Limosilactobacillus fermentum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
assay at Limosilactobacillus fermentum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
assay at Limosilactobacillus fermentum