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Literature summary for 3.5.1.5 extracted from

  • Martin, P.R.; Hausinger, R.P.
    Site-directed mutagenesis of the active site cysteine in Klebsiella aerogenes urease (1992), J. Biochem., 267, 20024-20027.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
C319A no activity Klebsiella aerogenes
C319A 48% of the activity of the wild-type enzyme, nickel content of the mutant is lower than that of the wild-type enzyme, small increase in Km-value Klebsiella aerogenes
C319D 0.03% of the activity of the wild-type enzyme, nickel content of the mutant is lower than that of the wild-type enzyme, small increase in Km-value Klebsiella aerogenes
C319S 4.5% of the activity of the wild-type enzyme, nickel content of the mutant is lower than that of the wild-type enzyme, small increase in Km-value Klebsiella aerogenes

Inhibitors

Inhibitors Comment Organism Structure
iodoacetamide phosphate protects wild-type enzyme from inactivation, does not affect inactivation of C319S urease Klebsiella aerogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.9
-
Urea wild-type enzyme Klebsiella aerogenes
5
-
Urea mutant 319A Klebsiella aerogenes
5.4
-
Urea mutant C319D Klebsiella aerogenes
11.3
-
Urea mutant C319S Klebsiella aerogenes

Metals/Ions

Metals/Ions Comment Organism Structure
Nickel wild-type enzyme contains 4.6 atoms of nickel per molecule of urease, the nickel content of the mutant enzymes C319A, C319S, C319D and C319Y is lower Klebsiella aerogenes
Nickel nickel metalloenzyme Klebsiella aerogenes

Organism

Organism UniProt Comment Textmining
Klebsiella aerogenes
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and mutant enzymes C319A, C319S, C319D and C319Y Klebsiella aerogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
urea + H2O
-
Klebsiella aerogenes CO2 + NH3
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2
-
mutant enzymes C319S and C319D Klebsiella aerogenes
6.7
-
mutant C319A Klebsiella aerogenes
7.8
-
wild-type enzyme Klebsiella aerogenes