Protein Variants | Comment | Organism |
---|---|---|
C319A | no activity | Klebsiella aerogenes |
C319A | 48% of the activity of the wild-type enzyme, nickel content of the mutant is lower than that of the wild-type enzyme, small increase in Km-value | Klebsiella aerogenes |
C319D | 0.03% of the activity of the wild-type enzyme, nickel content of the mutant is lower than that of the wild-type enzyme, small increase in Km-value | Klebsiella aerogenes |
C319S | 4.5% of the activity of the wild-type enzyme, nickel content of the mutant is lower than that of the wild-type enzyme, small increase in Km-value | Klebsiella aerogenes |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | phosphate protects wild-type enzyme from inactivation, does not affect inactivation of C319S urease | Klebsiella aerogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.9 | - |
Urea | wild-type enzyme | Klebsiella aerogenes | |
5 | - |
Urea | mutant 319A | Klebsiella aerogenes | |
5.4 | - |
Urea | mutant C319D | Klebsiella aerogenes | |
11.3 | - |
Urea | mutant C319S | Klebsiella aerogenes |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Nickel | wild-type enzyme contains 4.6 atoms of nickel per molecule of urease, the nickel content of the mutant enzymes C319A, C319S, C319D and C319Y is lower | Klebsiella aerogenes | |
Nickel | nickel metalloenzyme | Klebsiella aerogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella aerogenes | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and mutant enzymes C319A, C319S, C319D and C319Y | Klebsiella aerogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
urea + H2O | - |
Klebsiella aerogenes | CO2 + NH3 | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.2 | - |
mutant enzymes C319S and C319D | Klebsiella aerogenes |
6.7 | - |
mutant C319A | Klebsiella aerogenes |
7.8 | - |
wild-type enzyme | Klebsiella aerogenes |