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Literature summary for 3.5.1.5 extracted from

  • Lee, S.G.; Calhoun, D.H.
    Urease from a potentially pathogenic coccoid isolate: purification, characzterization, and comparison to other microbial ureases (1997), Infect. Immun., 65, 3991-3996.
    View publication on PubMedView publication on EuropePMC

General Stability

General Stability Organism
unstable during purification in absence of nickel. When 0.005 mM NiSO4 is present during growth and at 0.1 mM in buffers during sonication and purification, the urease is completely stable at room temperature during the purification procedure bacterium strain SL100

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7
-
Urea
-
bacterium strain SL100

Metals/Ions

Metals/Ions Comment Organism Structure
Nickel nickel metalloenzyme bacterium strain SL100

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21000
-
3 * 21000 + 3 * 65000, SDS-PAGE bacterium strain SL100
65000
-
3 * 21000 + 3 * 65000, SDS-PAGE bacterium strain SL100
260000
-
gel filtration bacterium strain SL100

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
urea + H2O bacterium strain SL100 urease may be a virulence factor during colonization of the stomach ?
-
?

Organism

Organism UniProt Comment Textmining
bacterium strain SL100
-
gram-positive coccoid isolate prototype with an adhesin specific for gastric mucin
-

Purification (Commentary)

Purification (Comment) Organism
-
bacterium strain SL100

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1120
-
-
bacterium strain SL100

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
urea + H2O
-
bacterium strain SL100 CO2 + NH3
-
?
urea + H2O urease may be a virulence factor during colonization of the stomach bacterium strain SL100 ?
-
?

Subunits

Subunits Comment Organism
hexamer 3 * 21000 + 3 * 65000, SDS-PAGE bacterium strain SL100

pH Stability

pH Stability pH Stability Maximum Comment Organism
1.3
-
30 min, retains most of its activity bacterium strain SL100