General Stability | Organism |
---|---|
unstable during purification in absence of nickel. When 0.005 mM NiSO4 is present during growth and at 0.1 mM in buffers during sonication and purification, the urease is completely stable at room temperature during the purification procedure | bacterium strain SL100 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.7 | - |
Urea | - |
bacterium strain SL100 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Nickel | nickel metalloenzyme | bacterium strain SL100 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21000 | - |
3 * 21000 + 3 * 65000, SDS-PAGE | bacterium strain SL100 |
65000 | - |
3 * 21000 + 3 * 65000, SDS-PAGE | bacterium strain SL100 |
260000 | - |
gel filtration | bacterium strain SL100 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
urea + H2O | bacterium strain SL100 | urease may be a virulence factor during colonization of the stomach | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
bacterium strain SL100 | - |
gram-positive coccoid isolate prototype with an adhesin specific for gastric mucin | - |
Purification (Comment) | Organism |
---|---|
- |
bacterium strain SL100 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1120 | - |
- |
bacterium strain SL100 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
urea + H2O | - |
bacterium strain SL100 | CO2 + NH3 | - |
? | |
urea + H2O | urease may be a virulence factor during colonization of the stomach | bacterium strain SL100 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 3 * 21000 + 3 * 65000, SDS-PAGE | bacterium strain SL100 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
1.3 | - |
30 min, retains most of its activity | bacterium strain SL100 |