KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12.5 | - |
Urea | - |
Pseudarthrobacter oxydans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Nickel | nickel metalloenzyme | Pseudarthrobacter oxydans | |
Nickel | specifically required for ureolysis and cannot be replaced by another metal, 0.5 mol of nickel is firmly bound to 1 mol of enzyme protein | Pseudarthrobacter oxydans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
242000 | - |
gel filtration | Pseudarthrobacter oxydans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudarthrobacter oxydans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudarthrobacter oxydans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
urea + H2O | - |
Pseudarthrobacter oxydans | CO2 + NH3 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Pseudarthrobacter oxydans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
stable up to | Pseudarthrobacter oxydans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
- |
Pseudarthrobacter oxydans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
and below, nickel is irreversibly removed with concommitant loss of enzyme activity | Pseudarthrobacter oxydans |
7 | - |
in presence of 10 mM EDTA protein-nickel binding remains intact | Pseudarthrobacter oxydans |