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Literature summary for 3.5.1.46 extracted from

  • Taguchi, H.; Wakamatsu, M.; Aso, K.; Ono, S.; Shin, T.; Akamatsu, T.
    A simple assay for 6-aminohexanoate-oligomer-hydrolase using N-(4-nitrophenyl)-6-aminohexanamide (2012), J. Biosci. Bioeng., 114, 586-588.
    View publication on PubMed
Search for more literature for 3.5.1.46

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas sp.
expression in Escherichia coli Arthrobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
 N-(4-nitrophenyl)-6-aminohexanamide pH 7.3, 30°C, cell free extract Arthrobacter sp.
2
-
 N-(4-nitrophenyl)-6-aminohexanamide pH 7.3, 30°C, cell free extract Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-(6-aminohexanoyl)-6-aminohexanoate + H2O Pseudomonas sp. the enzyme is involved degradation of nylon-6 2 6-aminohexanoate
-
 ?
N-(6-aminohexanoyl)-6-aminohexanoate + H2O Arthrobacter sp. the enzyme is involved degradation of nylon-6 2 6-aminohexanoate
-
 ?
N-(6-aminohexanoyl)-6-aminohexanoate + H2O Pseudomonas sp. NK87 the enzyme is involved degradation of nylon-6 2 6-aminohexanoate
-
 ?
N-(6-aminohexanoyl)-6-aminohexanoate + H2O Arthrobacter sp. KI72 the enzyme is involved degradation of nylon-6 2 6-aminohexanoate
-
 ?

Organism

Organism UniProt Comment Textmining
Arthrobacter sp.
-
-
-
Arthrobacter sp. KI72
-
-
-
Pseudomonas sp.
-
-
-
Pseudomonas sp. NK87
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-(4-nitrophenyl)-6-aminohexanamide + H2O
-
 Pseudomonas sp. 4-nitroaniline + 6-aminohexanoate
-
 ?
N-(4-nitrophenyl)-6-aminohexanamide + H2O
-
 Arthrobacter sp. 4-nitroaniline + 6-aminohexanoate
-
 ?
N-(4-nitrophenyl)-6-aminohexanamide + H2O
-
 Pseudomonas sp. NK87 4-nitroaniline + 6-aminohexanoate
-
 ?
N-(4-nitrophenyl)-6-aminohexanamide + H2O
-
 Arthrobacter sp. KI72 4-nitroaniline + 6-aminohexanoate
-
 ?
N-(6-aminohexanoyl)-6-aminohexanoate + H2O
-
 Pseudomonas sp. 2 6-aminohexanoate
-
 ?
N-(6-aminohexanoyl)-6-aminohexanoate + H2O
-
 Arthrobacter sp. 2 6-aminohexanoate
-
 ?
N-(6-aminohexanoyl)-6-aminohexanoate + H2O the enzyme is involved degradation of nylon-6 Pseudomonas sp. 2 6-aminohexanoate
-
 ?
N-(6-aminohexanoyl)-6-aminohexanoate + H2O the enzyme is involved degradation of nylon-6 Arthrobacter sp. 2 6-aminohexanoate
-
 ?
N-(6-aminohexanoyl)-6-aminohexanoate + H2O
-
 Pseudomonas sp. NK87 2 6-aminohexanoate
-
 ?
N-(6-aminohexanoyl)-6-aminohexanoate + H2O the enzyme is involved degradation of nylon-6 Pseudomonas sp. NK87 2 6-aminohexanoate
-
 ?
N-(6-aminohexanoyl)-6-aminohexanoate + H2O
-
 Arthrobacter sp. KI72 2 6-aminohexanoate
-
 ?
N-(6-aminohexanoyl)-6-aminohexanoate + H2O the enzyme is involved degradation of nylon-6 Arthrobacter sp. KI72 2 6-aminohexanoate
-
 ?

Synonyms

Synonyms Comment Organism
NylB
-
 Pseudomonas sp.
NylB
-
 Arthrobacter sp.