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Literature summary for 3.5.1.41 extracted from

  • Liu, L.; Qu, M.; Liu, T.; Chen, Q.; Guo, X.; Yang, J.; Yang, Q.
    Biochemical characterization of three midgut chitin deacetylases of the Lepidopteran insect Bombyx mori (2019), J. Insect Physiol., 113, 42-48 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
CDA6 isozyme DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Pichia pastoris strain GS115, quantitative real-time PCR expression analysis and isozyme expression patterns Bombyx mori
CDA7 isozyme DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Pichia pastoris strain GS115, quantitative real-time PCR expression analysis and isozyme expression patterns Bombyx mori
CDA8 isozyme DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Pichia pastoris strain GS115, quantitative real-time PCR expression analysis and isozyme expression patterns Bombyx mori

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
colloidal chitin + H2O Bombyx mori
-
?
-
?
peritrophic matrix chitin + H2O Bombyx mori
-
?
-
?

Organism

Organism UniProt Comment Textmining
Bombyx mori H9JW43
-
-
Bombyx mori H9JW44
-
-
Bombyx mori H9JW45
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged isozyme BmCDA6 from Pichia pastoris strain GS115 by ammonium sulfate fractionation, desalting gel filtration, and nickel affinity chromatography Bombyx mori
recombinant His-tagged isozyme BmCDA7 from Pichia pastoris strain GS115 by ammonium sulfate fractionation, desalting gel filtration, and nickel affinity chromatography Bombyx mori
recombinant His-tagged isozyme BmCDA8 from Pichia pastoris strain GS115 by ammonium sulfate fractionation, desalting gel filtration, and nickel affinity chromatography Bombyx mori

Source Tissue

Source Tissue Comment Organism Textmining
midgut
-
Bombyx mori
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
enzymatic activity as micromol acetate/min/micromol of protein Bombyx mori
0.061
-
substrate peritrophic matrix chitin, isozyme BmCDA8, pH 7.4, 30°C Bombyx mori
0.078
-
substrate colloidal chitin, isozyme BmCDA6, pH 7.4, 30°C Bombyx mori
0.207
-
substrate colloidal chitin, isozyme BmCDA8, pH 7.4, 30°C Bombyx mori
0.284
-
substrate peritrophic matrix chitin, isozyme BmCDA7, pH 7.4, 30°C Bombyx mori
0.393
-
substrate colloidal chitin, isozyme BmCDA7, pH 7.4, 30°C Bombyx mori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
colloidal chitin + H2O
-
Bombyx mori ?
-
?
additional information no activity with peritrophic matrix chitin by isozyme BmCDA6 Bombyx mori ?
-
?
peritrophic matrix chitin + H2O
-
Bombyx mori ?
-
?

Subunits

Subunits Comment Organism
? x * 41400, about, isozyme BmCDA7 , sequence calculation Bombyx mori
? x * 41500, about, isozyme BmCDA8 , sequence calculation Bombyx mori
? x * 41700, about, isozyme BmCDA6, sequence calculation Bombyx mori

Synonyms

Synonyms Comment Organism
BmCDA6
-
Bombyx mori
BmCDA7
-
Bombyx mori
BmCDA8
-
Bombyx mori
CDA
-
Bombyx mori
midgut chitin deacetylase
-
Bombyx mori
NodB homology domain-containing protein UniProt Bombyx mori

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Bombyx mori

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Bombyx mori

pI Value

Organism Comment pI Value Maximum pI Value
Bombyx mori sequence calculation
-
5.13
Bombyx mori sequence calculation
-
5.21
Bombyx mori sequence calculation
-
5.71