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Literature summary for 3.5.1.41 extracted from

  • Yu, R.; Liu, W.; Li, D.; Zhao, X.; Ding, G.; Zhang, M.; Ma, E.; Zhu, K.; Li, S.; Moussian, B.; Zhang, J.
    Helicoidal organization of chitin in the cuticle of the migratory locust requires the function of the chitin deacetylase2 enzyme (LmCDA2) (2016), J. Biol. Chem., 291, 24352-24363 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.41

Cloned(Commentary)

Cloned (Comment) Organism
gene CDA2a, two splicing variants a and b exist, sequence comparisons, gene structure analysis, and phylogenetic analysis and tree, recombinant expression in Escherichia coli Locusta migratoria
gene CDA2b, two splicing variants a and b exist, sequence comparisons, gene structure analysis, and phylogenetic analysis and tree, recombinant expression in Escherichia coli Locusta migratoria

Protein Variants

Protein Variants Comment Organism
additional information LmCDA2 expression is knocked down by RNA interference (RNAi), injection of dsRNA against LmCDA2 into 2-day-old fifth instar nymphs. The dsLmCDA2-injected nymphs fail to shed the old cuticle and are trapped within the exuviae until they died. Their mortality is 80% Locusta migratoria

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular enzyme LmCDA2 localizes only to the initially produced chitin microfibrils that constitute the apical site of the chitin stack Locusta migratoria
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Organism

Organism UniProt Comment Textmining
Locusta migratoria A0A2R2PTF2
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Locusta migratoria A0A2R2PTF4
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Source Tissue

Source Tissue Comment Organism Textmining
foregut
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 Locusta migratoria
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additional information both isozymes LmCDA2a and LmCDA2b have the highest expression levels in the foregut, while their expression is low in the other tissues tested. Stage-dependent expression pattern of LmCDA2, mRNA expression levels in the integument at days 1-7 of fifth-instar nymphs are monitored by quantitative RT-PCR expression analysis Locusta migratoria
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Synonyms

Synonyms Comment Organism
CDA2
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 Locusta migratoria
chitin deacetylase2
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 Locusta migratoria
LmCDA2
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 Locusta migratoria
LmCDA2a
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 Locusta migratoria
LmCDA2b
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 Locusta migratoria

General Information

General Information Comment Organism
malfunction LmCDA2-deficient cuticle is less compact suggesting that LmCDA2 is needed for chitin packaging. Animals with reduced LmCDA2 activity die at molting, underlining that correct chitin organization is essential for survival Locusta migratoria
physiological function LmCDA2-mediated chitin deacetylation at the beginning of chitin production is a decisive reaction that triggers helicoidal arrangement of subsequently assembled chitin-protein microfibrils. In the body cuticle of nymphs of the migratory locust Locusta migratoria, helicoidal chitin organization is changed to an organization with unidirectional microfibril orientation. The enzyme is involved in cuticle organization. LmCDA2 is needed for tracheal cuticle formation and feeding, and LmCDA2 is required for locust molting and development Locusta migratoria