Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetate | the enzyme is strongly inhibited by acetate with a concentration higher than 1 mM | Lichtheimia corymbifera | |
Ag+ | 70% residual activity at 10 mM | Lichtheimia corymbifera | |
Cu2+ | 14% residual activity at 10 mM | Lichtheimia corymbifera | |
EDTA | complete inhibition at 10 mM | Lichtheimia corymbifera | |
Fe2+ | 10% residual activity at 10 mM | Lichtheimia corymbifera | |
Fe3+ | 80% residual activity at 10 mM | Lichtheimia corymbifera | |
Hg2+ | complete inhibition at 10 mM | Lichtheimia corymbifera | |
Ni2+ | 24% residual activity at 10 mM | Lichtheimia corymbifera | |
Pb2+ | 6% residual activity at 10 mM | Lichtheimia corymbifera | |
Sn2+ | complete inhibition at 10 mM | Lichtheimia corymbifera | |
Zn2+ | 30% residual activity at 10 mM | Lichtheimia corymbifera |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Lichtheimia corymbifera | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 164% activity at 1 mM | Lichtheimia corymbifera | |
Co2+ | 184% activity at 10 mM | Lichtheimia corymbifera | |
Mg2+ | 130% activity at 1 or 10 mM | Lichtheimia corymbifera | |
Mn2+ | 117% activity at 10 mM | Lichtheimia corymbifera |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lichtheimia corymbifera | - |
- |
- |
Lichtheimia corymbifera DY-9 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitin + H2O | 19% activity compared to WSCT-50 | Lichtheimia corymbifera | chitosan + acetate | - |
? | |
chitin + H2O | 19% activity compared to WSCT-50 | Lichtheimia corymbifera DY-9 | chitosan + acetate | - |
? | |
CM chitin + H2O | 8% degree of deacetylation with 13% activity compared to water-soluble chitin with degree of deacetylation 50% | Lichtheimia corymbifera | ? | - |
? | |
crab chitosan + H2O | 71-88% degree of deacetylation with 21-10% activity compared to water-soluble chitin with degree of deacetylation 50% | Lichtheimia corymbifera | ? | - |
? | |
di-N-acetylchitobiose + H2O | 35% activity compared to hepta-N-acetylchitoheptaose | Lichtheimia corymbifera | ? | - |
? | |
di-N-acetylchitobiose + H2O | 35% activity compared to hepta-N-acetylchitoheptaose | Lichtheimia corymbifera DY-9 | ? | - |
? | |
hepta-N-acetylchitoheptaose + H2O | 100% activity | Lichtheimia corymbifera | ? | - |
? | |
hexa-N-acetylchitohexaose + H2O | 95.3% activity compared to hepta-N-acetylchitoheptaose | Lichtheimia corymbifera | ? | - |
? | |
hexa-N-acetylchitohexaose + H2O | 95.3% activity compared to hepta-N-acetylchitoheptaose | Lichtheimia corymbifera DY-9 | ? | - |
? | |
additional information | GlcNAc, colloidal chitin, regenerated chitin, beta-chitin and crystal crab chitins with different particle size are not deacetylated by the crude enzyme | Lichtheimia corymbifera | ? | - |
? | |
additional information | GlcNAc, colloidal chitin, regenerated chitin, beta-chitin and crystal crab chitins with different particle size are not deacetylated by the crude enzyme | Lichtheimia corymbifera DY-9 | ? | - |
? | |
penta-N-acetylchitopentaose + H2O | 75.7% activity compared to hepta-N-acetylchitoheptaose | Lichtheimia corymbifera | ? | - |
? | |
penta-N-acetylchitopentaose + H2O | 75.7% activity compared to hepta-N-acetylchitoheptaose | Lichtheimia corymbifera DY-9 | ? | - |
? | |
swollen chitin + H2O | 8% degree of deacetylation with 6% activity compared to water-soluble chitin with degree of deacetylation 50% | Lichtheimia corymbifera | ? | - |
? | |
tetra-N-acetylchitotetraose + H2O | 41.8% activity compared to hepta-N-acetylchitoheptaose | Lichtheimia corymbifera | ? | - |
? | |
tri-N-acetylchitotriose + H2O | 38.9% activity compared to hepta-N-acetylchitoheptaose | Lichtheimia corymbifera | ? | - |
? | |
WSCT-50 + H2O | 100% activity, water-soluble chitin with degree of deacetylation 50% | Lichtheimia corymbifera | ? + acetate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CDA | - |
Lichtheimia corymbifera |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Lichtheimia corymbifera |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 45 | the enzyme is stable below 40°C but the activity significantly declines above 45°C | Lichtheimia corymbifera |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Lichtheimia corymbifera |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | - |
Lichtheimia corymbifera |
Organism | Comment | Expression |
---|---|---|
Lichtheimia corymbifera | the highest yield of enzyme is achieved in a liquid medium containing 2% powder chitin at pH 9.0, 28°C and 150 rpm | up |