Literature summary for 3.5.1.41 extracted from

Wang, Y.; Song, J.Z.; Yang, Q.; Liu, Z.H.; Huang, X.M.; Chen, Y.
Cloning of a heat-stable chitin deacetylase gene from Aspergillus nidulans and its functional expression in Escherichia coli (2010), Appl. Biochem. Biotechnol., 162, 843-854.

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Cloned(Commentary)

Cloned (Comment)	Organism
the chitin deacetylase gene is subcloned into a pET28a expression vector and expressed in Escherichia coli BL21 cells	Aspergillus nidulans

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	-	Aspergillus nidulans

Organism

Organism	UniProt	Comment	Textmining
Aspergillus nidulans	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
His-Bind column chromatography	Aspergillus nidulans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	the specific activity using glycol chitin as substrate is 4.17 units/mg	Aspergillus nidulans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
glycol chitin + H2O	-	Aspergillus nidulans	chitosan + acetate	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	purified enzyme	Aspergillus nidulans

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
6.25	-	glycol chitin	purified enzyme, at pH 8.0 and 50°C	Aspergillus nidulans

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	purified enzyme	Aspergillus nidulans

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Release 2023.1 (January 2023)