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Literature summary for 3.5.1.4 extracted from

  • Wang, J.; Zhu, Y.; Zhao, G.; Zhu, J.; Wu, S.
    Characterization of a recombinant (+)-gamma-lactamase from Microbacterium hydrocarbonoxydans which provides evidence that two enantiocomplementary gamma-lactamases are in the strain (2015), Appl. Microbiol. Biotechnol., 99, 3069-3080.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Microbacterium hydrocarbonoxydans

Protein Variants

Protein Variants Comment Organism
C118A complete loss of activity Microbacterium hydrocarbonoxydans
D9A complete loss of activity Microbacterium hydrocarbonoxydans
H51A complete loss of activity Microbacterium hydrocarbonoxydans
K84A 9% decrease in activity compared to wild-type Microbacterium hydrocarbonoxydans
Q11A 20% decrease in activity compared to wild-type Microbacterium hydrocarbonoxydans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19700
-
2 * 19700, calculated, 2 * 20000, SDS-PAGE Microbacterium hydrocarbonoxydans
20000
-
2 * 19700, calculated, 2 * 20000, SDS-PAGE Microbacterium hydrocarbonoxydans
39000
-
gel filtration Microbacterium hydrocarbonoxydans
40000
-
PAGE Microbacterium hydrocarbonoxydans

Organism

Organism UniProt Comment Textmining
Microbacterium hydrocarbonoxydans A0A077AY47 additionally acts as isochorismatase, EC 3.3.2.1 and as (+)-gamma-lactamase
-
Microbacterium hydrocarbonoxydans E3SVS0 additionally acts as as (+)-gamma-lactamase
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-lactamide + H2O about 30% of the activity with (S)-lactamide Microbacterium hydrocarbonoxydans lactate + NH3
-
?
(S)-lactamide + H2O
-
Microbacterium hydrocarbonoxydans lactate + NH3
-
?
(S)-lactamide + H2O about 35% of the activity with propionamide Microbacterium hydrocarbonoxydans lactate + NH3
-
?
acetamide + H2O about 10% of the activity with propionamide Microbacterium hydrocarbonoxydans acetate + NH3
-
?
acetamide + H2O about 25% of the activity with (S)-lactamide Microbacterium hydrocarbonoxydans acetate + NH3
-
?
propionamide + H2O
-
Microbacterium hydrocarbonoxydans propionic acid + NH3
-
?
propionamide + H2O about 50% of the activity with (S)-lactamide Microbacterium hydrocarbonoxydans propionic acid + NH3
-
?
R-lactamide + H2O about 65% of the activity with propionamide Microbacterium hydrocarbonoxydans lactate + NH3
-
?

Subunits

Subunits Comment Organism
dimer 2 * 19700, calculated, 2 * 20000, SDS-PAGE Microbacterium hydrocarbonoxydans

Synonyms

Synonyms Comment Organism
mHG
-
Microbacterium hydrocarbonoxydans
Mhpg
-
Microbacterium hydrocarbonoxydans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Microbacterium hydrocarbonoxydans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
30 min, 38% reidual activity Microbacterium hydrocarbonoxydans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Microbacterium hydrocarbonoxydans

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 10 stable within Microbacterium hydrocarbonoxydans