Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | presence of mid-chain aliphatic and amino acid amides enhances the enzymatic activity | Geobacillus subterraneus |
Cloned (Comment) | Organism |
---|---|
constitutive enzyme | Geobacillus subterraneus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | complete inhibition at 1 mM | Geobacillus subterraneus | |
Ba2+ | 11% inhibition at 1 mM | Geobacillus subterraneus | |
Ca2+ | 10% inhibition at 1 mM | Geobacillus subterraneus | |
Co2+ | 9% inhibition at 1 mM | Geobacillus subterraneus | |
Cu2+ | 28.5% inhibition at 1 mM | Geobacillus subterraneus | |
DTT | 21% inhibition at 1 mM | Geobacillus subterraneus | |
EDTA | 21% inhibition at 1 mM | Geobacillus subterraneus | |
Fe3+ | 87% inhibition at 1 mM | Geobacillus subterraneus | |
Hg2+ | complete inhibition at 1 mM | Geobacillus subterraneus | |
Mg2+ | 53% inhibition at 1 mM | Geobacillus subterraneus | |
Mn2+ | 12% inhibition at 1 mM | Geobacillus subterraneus | |
Na+ | 17% inhibition at 1 mM | Geobacillus subterraneus | |
Pb2+ | 8% inhibition at 1 mM | Geobacillus subterraneus | |
PEG | 8% inhibition at 1 mM | Geobacillus subterraneus | |
PMSF | 87.5% inhibition at 1 mM | Geobacillus subterraneus | |
Zn2+ | 11% inhibition at 1 mM | Geobacillus subterraneus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | MichaelisMenten kinetics | Geobacillus subterraneus | |
6.02 | - |
nicotinamide | pH 6.5, 70°C | Geobacillus subterraneus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
3 * 37000, SDS-PAGE | Geobacillus subterraneus |
111000 | - |
gel filtration | Geobacillus subterraneus |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | 5-10% activity remaining at 5-10% acetone after 15 min | Geobacillus subterraneus |
benzene | 70-80% activity remaining at 5-10% benzene after 15 min | Geobacillus subterraneus |
Butanol | 15-25% activity remaining at 5-10% butanol after 15 min | Geobacillus subterraneus |
CCl4 | 80-90% activity remaining at 5-10% CCl4 after 15 min | Geobacillus subterraneus |
dimethyl sulfoxide | 35-40% activity remaining at 5-10% DMSO after 15 min | Geobacillus subterraneus |
Ethanol | 15% activity remaining at 5-10% ethanol after 15 min | Geobacillus subterraneus |
Ethylene glycol | 60-70% activity remaining at 5-10% ethylene glycol after 15 min | Geobacillus subterraneus |
propanol | 20-25% activity remaining at 5-10% propanol after 15 min | Geobacillus subterraneus |
toluene | 90% activity remaining at 10% toluene after 15 min | Geobacillus subterraneus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus subterraneus | - |
isolated from Manikaran thermal spring (Himachal Pradesh, India) | - |
Geobacillus subterraneus RL-2a | - |
isolated from Manikaran thermal spring (Himachal Pradesh, India) | - |
Purification (Comment) | Organism |
---|---|
native enzyme 52.04fold to homogeneity by heat treatment, ammonium sulfate fractionation, anion exchange chromatography, and gel filtration | Geobacillus subterraneus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | constitutive enzyme | Geobacillus subterraneus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
48.66 | - |
purified native enzyme, pH 6.5, 70°C, substrate nicotinamide | Geobacillus subterraneus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzamide + H2O | - |
Geobacillus subterraneus | benzoate + NH3 | - |
? | |
butyramide + H2O | high activity | Geobacillus subterraneus | butyrate + NH3 | - |
? | |
cyanoacetamide + H2O | low activity | Geobacillus subterraneus | cyanoacetate + NH3 | - |
? | |
formamide + H2O | - |
Geobacillus subterraneus | formate + NH3 | - |
? | |
formamide + H2O | - |
Geobacillus subterraneus RL-2a | formate + NH3 | - |
? | |
isobutyramide + H2O | high activity | Geobacillus subterraneus | isobutyrate + NH3 | - |
? | |
isobutyramide + H2O | high activity | Geobacillus subterraneus RL-2a | isobutyrate + NH3 | - |
? | |
isonicotinamide + H2O | - |
Geobacillus subterraneus | isonicotinate + NH3 | - |
? | |
L-leucinamide + H2O | - |
Geobacillus subterraneus | L-leucine + NH3 | - |
? | |
L-leucinamide + H2O | - |
Geobacillus subterraneus RL-2a | L-leucine + NH3 | - |
? | |
lactamide + H2O | - |
Geobacillus subterraneus | lactate + NH3 | - |
? | |
methacrylamide + H2O | - |
Geobacillus subterraneus | methacrylate + NH3 | - |
? | |
additional information | glycinamide, malonamide, acetamide, and acrylamide are poor substrates, while urea, mandelamide, N-methylacetamide, and thioacetamide are no substrates, substrate specificity, overview | Geobacillus subterraneus | ? | - |
? | |
additional information | glycinamide, malonamide, acetamide, and acrylamide are poor substrates, while urea, mandelamide, N-methylacetamide, and thioacetamide are no substrates, substrate specificity, overview | Geobacillus subterraneus RL-2a | ? | - |
? | |
nicotinamide + H2O | reaction of EC 3.5.1.19, nicotinamidase | Geobacillus subterraneus | nicotinate + NH3 | - |
? | |
prolinamide + H2O | - |
Geobacillus subterraneus | proline + NH3 | - |
? | |
propainamide + H2O | high activity | Geobacillus subterraneus | ? + NH3 | - |
? | |
propionamide + H2O | best substrate | Geobacillus subterraneus | propionate + NH3 | - |
? | |
sulfanilamide + H2O | - |
Geobacillus subterraneus | sulfanilate + NH3 | - |
? | |
valeramide + H2O | high activity | Geobacillus subterraneus | valerate + NH3 | - |
? | |
valeramide + H2O | high activity | Geobacillus subterraneus RL-2a | valerate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 37000, SDS-PAGE | Geobacillus subterraneus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Geobacillus subterraneus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 90 | activity range | Geobacillus subterraneus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
purified native enzyme, half-life is 354 min | Geobacillus subterraneus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Geobacillus subterraneus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 11.5 | activity range | Geobacillus subterraneus |
General Information | Comment | Organism |
---|---|---|
evolution | peptide sequences determination by MALDI-TOF analysis reveals 97% similarity with that of glutamyl-tRNA(Gln) amidotransferase subunit A (Geobacillus kaustophilus HTA426) and amidase of Geobacillus sp. C56-T3, also 68% similarity to amidase of Geobacillus sp. Y412MC61 | Geobacillus subterraneus |
additional information | the enzyme is a wide-spectrum thermostable amidase | Geobacillus subterraneus |