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Literature summary for 3.5.1.4 extracted from

  • Park, H.J.; Uhm, K.N.; Kim, H.K.
    Biotransformation of amides to acids using a co-cross-linked enzyme aggregate of Rhodococcus erythropolis amidase (2010), J. Microbiol. Biotechnol., 20, 325-331.
    View publication on PubMed
Search for more literature for 3.5.1.4

Application

Application Comment Organism
industry the immobilized, cross-linked enzyme aggregate proves useful as a substitute for soluble amidase as a biocatalyst in the pharmaceutical and chemical industries Rhodococcus erythropolis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain BL21(DE3) Rhodococcus erythropolis

Protein Variants

Protein Variants Comment Organism
additional information generation of an immobilized, cross-linked enzyme aggregate by mixing of the recombinant, crude, soluble enzyme with bovine serum albumin, and ammonium sulfate precipitation, followed by cross-linking of the resultant precipitant with glutaraldehyde. The enzyme aggregate shows hydrolytic activity toward a variety of amide substrates, and increased reaction stability Rhodococcus erythropolis

Organism

Organism UniProt Comment Textmining
Rhodococcus erythropolis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-chloro-3-hydroxybutyramide + H2O substrate of the soluble enzyme and of the immobilized, cross-linked enzyme Rhodococcus erythropolis 4-chloro-3-hydroxybutyrate + NH3
-
 ?
acetamide + H2O substrate of the soluble enzyme and of the immobilized, cross-linked enzyme, low activity Rhodococcus erythropolis acetate + NH3
-
 ?
acrylamide + H2O substrate of the soluble enzyme and of the immobilized, cross-linked enzyme Rhodococcus erythropolis acrylate + NH3
-
 ?
benzamide + H2O substrate of the soluble enzyme and of the immobilized, cross-linked enzyme Rhodococcus erythropolis benzoate + NH3
-
 ?
butyramide + H2O substrate of the soluble enzyme and of the immobilized, cross-linked enzyme Rhodococcus erythropolis butyrate + NH3
-
 ?
isobutyramide + H2O substrate of the soluble enzyme and of the immobilized, cross-linked enzyme, preferred substrate Rhodococcus erythropolis isobutyrate + NH3
-
 ?
propionamide + H2O substrate of the soluble enzyme and of the immobilized, cross-linked enzyme Rhodococcus erythropolis propionate + NH3
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 immobilized, cross-linked enzyme aggregate Rhodococcus erythropolis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 immobilized, cross-linked enzyme aggregate Rhodococcus erythropolis