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Literature summary for 3.5.1.4 extracted from
- R-stereoselective amidase from Rhodococcus erythropolis No. 7 acting on 4-chloro-3-hydroxybutyramide (2008), J. Microbiol. Biotechnol., 18, 552-559.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Rhodococcus erythropolis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| HgCl2 | - |
Rhodococcus erythropolis |  |
| iodoacetamide | - |
Rhodococcus erythropolis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | - |
Benzamide | - |
Rhodococcus erythropolis | |
| 0.18 | - |
Isobutyramide | - |
Rhodococcus erythropolis | |
| 0.18 | - |
butyramide | - |
Rhodococcus erythropolis | |
| 1.3 | - |
Propionamide | - |
Rhodococcus erythropolis | |
| 4.79 | - |
Acrylamide | - |
Rhodococcus erythropolis | |
| 5.57 | - |
acetamide | - |
Rhodococcus erythropolis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodococcus erythropolis | B4XEY3 | - |
- |
| Rhodococcus erythropolis No. 7 | B4XEY3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rhodococcus erythropolis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-chloro-3-hydroxybutyramide + H2O | - |
Rhodococcus erythropolis | 4-chloro-3-hydroxybutyric acid + NH3 | - |
? | |
| acetamide + H2O | also acyl transfer activity in the presence of hydroxylamine | Rhodococcus erythropolis | acetic acid + NH3 | - |
? | |
| acetamide + H2O | also acyl transfer activity in the presence of hydroxylamine | Rhodococcus erythropolis No. 7 | acetic acid + NH3 | - |
? | |
| acrylamide + H2O | also acyl transfer activity in the presence of hydroxylamine | Rhodococcus erythropolis | acrylic acid + NH3 | - |
? | |
| acrylamide + H2O | also acyl transfer activity in the presence of hydroxylamine | Rhodococcus erythropolis No. 7 | acrylic acid + NH3 | - |
? | |
| benzamide + H2O | also acyl transfer activity in the presence of hydroxylamine | Rhodococcus erythropolis | benzoic acid + NH3 | - |
? | |
| benzamide + H2O | also acyl transfer activity in the presence of hydroxylamine | Rhodococcus erythropolis No. 7 | benzoic acid + NH3 | - |
? | |
| butyramide + H2O | - |
Rhodococcus erythropolis | butyric acid + NH3 | - |
? | |
| isobutyramide + H2O | - |
Rhodococcus erythropolis | isobutyric acid + NH3 | - |
? | |
| additional information | enantioselectivity toward (R)-4-chloro-3-hydroxybutyramide: e.e value 52% (conversion yield 57%) | Rhodococcus erythropolis | ? | - |
? | |
| additional information | enantioselectivity toward (R)-4-chloro-3-hydroxybutyramide: e.e value 52% (conversion yield 57%) | Rhodococcus erythropolis No. 7 | ? | - |
? | |
| propionamide + H2O | also acyl transfer activity in the presence of hydroxylamine | Rhodococcus erythropolis | propionic acid + NH3 | - |
? | |
| propionamide + H2O | also acyl transfer activity in the presence of hydroxylamine | Rhodococcus erythropolis No. 7 | propionic acid + NH3 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| amidase | - |
Rhodococcus erythropolis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
substrate: isobutyramide, no activity in Tris-HCl buffer | Rhodococcus erythropolis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
up to 50°C, 30 min | Rhodococcus erythropolis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.21 | - |
acetamide | - |
Rhodococcus erythropolis | |
| 0.48 | - |
Benzamide | - |
Rhodococcus erythropolis | |
| 0.58 | - |
Acrylamide | - |
Rhodococcus erythropolis | |
| 2.04 | - |
butyramide | - |
Rhodococcus erythropolis | |
| 4.03 | - |
Propionamide | - |
Rhodococcus erythropolis | |
| 5.68 | - |
Isobutyramide | - |
Rhodococcus erythropolis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
substrate: isobutyramide, no activity in Tris-HCl buffer | Rhodococcus erythropolis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 12 | 30 min | Rhodococcus erythropolis |
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