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Literature summary for 3.5.1.4 extracted from

  • Karmali, A.; Pacheco, R.; Tata, R.; Brown, P.
    Substitutions of Thr-103-Ile and Trp-138-Gly in amidase from Pseudomonas aeruginosa are responsible for altered kinetic properties and enzyme instability (2001), Mol. Biotechnol., 17, 201-212.
    View publication on PubMed
Search for more literature for 3.5.1.4

Cloned(Commentary)

Cloned (Comment) Organism
-
 Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
E59V mutant catalytically inactive Pseudomonas aeruginosa
T103I mutant able to use acetanilide as a carbon source, less thermostable Pseudomonas aeruginosa
T103I-W138G mutant with increased instability and less themrmostable Pseudomonas aeruginosa
W138G mutant able to use acetanilide as a carbon source Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 6 * 35000, gel filtration, wild type Pseudomonas aeruginosa
38000
-
 mutant E59V, SDS-PAGE Pseudomonas aeruginosa
78000
-
 mutant E59V, gel filtration Pseudomonas aeruginosa
78000
-
 mutant E59V, native PAGE Pseudomonas aeruginosa
210000
-
 wild type, gel filtration Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
 mutant strain KLAM59
-
Pseudomonas aeruginosa
-
 mutant strain Ph1
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas aeruginosa
affinity chromatography on epoxy-activated Sepharose 6B-acetamide and gel filtration Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetamide + H2O mutant W138G and T103I Pseudomonas aeruginosa acetic acid + NH3
-
 ?
acrylamide + H2O mutant W138G Pseudomonas aeruginosa prop-2-enoic acid + NH3
-
 ?
p-nitroacetanilide + H2O mutant T103I Pseudomonas aeruginosa acetic acid + p-nitroaniline
-
 ?
p-nitrophenylacetamide + H2O mutant W138G and T103I-W138G Pseudomonas aeruginosa p-nitrophenylacetic acid + NH3
-
 ?
phenylacetamide + H2O mutant W138G and T103I-W138G Pseudomonas aeruginosa phenylacetate + NH3
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 38000, SDS-PAGE and native PAGE, mutant E59V Pseudomonas aeruginosa
hexamer 6 * 35000, gel filtration, wild type Pseudomonas aeruginosa