Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.1.4 extracted from

  • Hollaway, M.R.; Clarke, P.H.; Ticho, T.
    Chloroacetone as an active-site-directed inhibitor of the aliphatic amidase from Pseudomonas aeruginosa (1980), Biochem. J., 191, 811-826.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
Chloroacetone pH 8.66, 25°C, irreversible strong inhibition at concentrations of 0.1 mM and above, acetamide protects enzyme from inhibition and slowly recovers activity of inactivated enzyme Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.8
-
acetamide pH 8.66, 25°C Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
PAC142
-
Pseudomonas aeruginosa PAC142
-
PAC142
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and dialysis and gel filtration on DEAE-Sephadex A-50 Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetamide + H2O
-
Pseudomonas aeruginosa acetic acid + NH3
-
?
acetamide + H2O
-
Pseudomonas aeruginosa PAC142 acetic acid + NH3
-
?
ethyl acetate + H2O
-
Pseudomonas aeruginosa acetic acid + ethanol
-
?
ethyl acetate + H2O
-
Pseudomonas aeruginosa PAC142 acetic acid + ethanol
-
?
N-methylacetamide + H2O
-
Pseudomonas aeruginosa acetic acid + methaneamine
-
?
N-methylacetamide + H2O
-
Pseudomonas aeruginosa PAC142 acetic acid + methaneamine
-
?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.001
-
Chloroacetone pH 7.1, 25°C, irreversible Pseudomonas aeruginosa