Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli JM109 | Pseudomonas aeruginosa |
Crystallization (Comment) | Organism |
---|---|
hanging-drop technique at both 16 and 4°C, rhombohedral morphology | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
C166A | mutant catalytically inactive | Pseudomonas aeruginosa |
C166S | mutant catalytically inactive | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38441 | - |
alpha = beta = gamma = 75°, and space group, R3 or R32, quaternary structure determinated by gel filtration and SDS-PAGE, 6 * 38441 deduced from primary structure | Pseudomonas aeruginosa |
38441 | - |
6 * 38441, calculated from primary structure | Pseudomonas aeruginosa |
215000 | - |
PAGE | Pseudomonas aeruginosa |
221000 | - |
gel filtration Sephacryl S300HR | Pseudomonas aeruginosa |
221000 | - |
gel filtration on Sephracryl S300HR | Pseudomonas aeruginosa |
224000 | - |
gel filtration Sephacryl S400HR | Pseudomonas aeruginosa |
224000 | - |
gel filtration on Sephracryl S400HR | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
harboring pALJ30 and pALJ20 | - |
Escherichia coli JM109 | - |
harboring pALJ30 and pALJ20 | - |
Pseudomonas aeruginosa | - |
C166S and C166A mutants | - |
Pseudomonas aeruginosa | - |
mutant strain KLAM59 | - |
Subunits | Comment | Organism |
---|---|---|
hexamer | with most probably 32 symmetry, crystals of both wild-type and C166S amidase grow with identical, rhombohedral morphology. X-ray diffraction analysis established the unit cell dimensions, a = b = c = 84 A | Pseudomonas aeruginosa |
hexamer | alpha = beta = gamma = 75°, and space group, R3 or R32, quaternary structure determinated by gel filtration and SDS-PAGE, 6 * 38441 deduced from primary structure | Pseudomonas aeruginosa |
hexamer | 6 * 38441, calculated from primary structure | Pseudomonas aeruginosa |