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Literature summary for 3.5.1.4 extracted from

  • Thiery, A.; Maestracci, M.; Arnaud, A.; Galzy, P.
    Acyltransferase activity of the wide spectrum amidase of Brevibacterium sp. R312 (1986), J. Gen. Microbiol., 132, 2205-2208.
No PubMed abstract available
Search for more literature for 3.5.1.4

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
16
-
 propionic acid ethyl ester
-
 Brevibacterium sp.
18
-
 ethyl acetate
-
 Brevibacterium sp.
31
-
 acetamide
-
 Brevibacterium sp.
83
-
 acetate
-
 Brevibacterium sp.
88
-
 Propionamide
-
 Brevibacterium sp.
93
-
 Acrylamide
-
 Brevibacterium sp.
218
-
 ethyl prop-2-enoate
-
 Brevibacterium sp.
628
-
 propionate
-
 Brevibacterium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ethyl acetate + H2O Brevibacterium sp.
-
 ethanol + acetate
-
 ?
ethyl acrylate + H2O Brevibacterium sp.
-
 acrylate + ethanol
-
 ?
ethyl propionate + H2O Brevibacterium sp.
-
 ethanol + propionate
-
 ?

Organism

Organism UniProt Comment Textmining
Brevibacterium sp.
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 in the presence of hydroxylamine, the amidase would convert acetamide to acetohydroxamic acid rather than to ammonium acetate Brevibacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetamide + H2O
-
 Brevibacterium sp. acetate + NH3
-
 ?
acrylamide + H2O
-
 Brevibacterium sp. acrylate + NH3
-
 ?
ethyl acetate + H2O
-
 Brevibacterium sp. ethanol + acetate
-
 ?
ethyl acrylate + H2O
-
 Brevibacterium sp. acrylate + ethanol
-
 ?
ethyl propionate + H2O
-
 Brevibacterium sp. ethanol + propionate
-
 ?
propionamide + H2O
-
 Brevibacterium sp. propionate + NH3
-
 ?