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Literature summary for 3.5.1.4 extracted from

  • Wyndham, R.C.; Slater, J.H.
    A comparative study of acquired amidase activity in Pseudomaonas species (1986), J. Gen. Microbiol., 132, 2185-2204.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
iodoacetamide
-
Pseudomonas aeruginosa
iodoacetamide no effect of 2-chloropropionamide Pseudomonas putida
N-ethylmaleimide inhibition at 10 mM, C-amidase Pseudomonas putida
p-hydroxymercuribenzoate
-
Pseudomonas aeruginosa
p-hydroxymercuribenzoate inhibition at 10 mM, inhibits the C-amidase Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
80
-
2-Chloropropionamide A-amidase Pseudomonas putida
100
-
acetamide A-amidase Pseudomonas putida
206
-
2-Chloropropionamide strain PPW3, C-amidase Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
strain PPW3, C-amidase, determined by gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-chloropropionamide + H2O Pseudomonas putida
-
2-chloropropionate + NH3
-
?
2-chloropropionamide + H2O Pseudomonas putida PP3
-
2-chloropropionate + NH3
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
PAU3 and PAU5
-
Pseudomonas putida
-
and strain PPW3
-
Pseudomonas putida PP3
-
and strain PPW3
-

Purification (Commentary)

Purification (Comment) Organism
strain PPW3, C-amidase, ammonium sulfate fractionation, gel filtration, DEAE-Sephadex, preparative PAGE, purification factor 40 Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00003
-
growth substrate 2-monochloropropionic acid, 2-chloropropionamide-amidase activity, measured with 80 mM 2-chloropropionamide, strain PP3 Pseudomonas putida
0.00024
-
growth substrate succinate, 2-chloropropionamide-amidase activity, measured with 80 mM 2-chloropropionamide, strain PP3 Pseudomonas putida
0.00027
-
growth substrate 2-chloropropionamide, 2-chloropropionamide-amidase activity, measured with 14 mM 2-chloropropionamide, strain PP3 Pseudomonas putida
0.0017
-
growth substrate propionamide, 2-chloropropionamide-amidase activity, measured with 80 mM 2-chloropropionamide, strain PAU3 Pseudomonas aeruginosa
0.019
-
growth substrate succinate, 2-chloropropionamide-amidase activity, measured with 80 mM 2-chloropropionamide, strain PPW3 Pseudomonas putida
0.023
-
growth substrate 2-monochloropropionic acid, 2-chloropropionamide-amidase activity, measured with 80 mM 2-chloropropionamide, strain PPW3 Pseudomonas putida
0.031
-
growth substrate succinate, 2-chloropropionamide-amidase activity, measured with 80 mM 2-chloropropionamide, strain PAU5 Pseudomonas putida
0.039
-
growth substrate propionamide, 2-chloropropionamide-amidase activity, measured with 80 mM 2-chloropropionamide, strain PAU5 Pseudomonas putida
0.078
-
A-amidase, substrate was 2-chloropropionamide Pseudomonas putida
0.12
-
growth substrate 2-chloropropionamide, 2-chloropropionamide-amidase activity, measured with 14 mM 2-chloropropionamide, strain PPW3 Pseudomonas putida
0.14
-
growth substrate 2-monochloropropionic acid, 2-chloropropionamide-amidase activity, measured with 80 mM 2-chloropropionamide, strain PAU5 Pseudomonas putida
0.25
-
growth substrate 2-chloropropionamide, 2-chloropropionamide-amidase activity, measured with 14 mM 2-chloropropionamide, strain PAU5 Pseudomonas putida
0.914
-
strain PPW, activity of C-amidase after preparative PAGE, final yield 17% of original activity Pseudomonas putida
1.89
-
strain PPW3, C-amidase, substrate 2-chloropropionamide Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-chloropropionamide + H2O
-
Pseudomonas putida 2-chloropropionate + NH3
-
?
2-chloropropionamide + H2O
-
Pseudomonas putida PP3 2-chloropropionate + NH3
-
?
acetamide + H2O
-
Pseudomonas putida acetate + NH3
-
?
acetamide + H2O
-
Pseudomonas putida PP3 acetate + NH3
-
?

Subunits

Subunits Comment Organism
More strain PPW3, C-amidase, subunit molecular mass of 29000 Da, SDS-PAGE Pseudomonas putida

Synonyms

Synonyms Comment Organism
A-amidase specificity towards aliphatic amides Pseudomonas aeruginosa
C-amidase specificity towards chlorinated aliphatic amides Pseudomonas putida