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Literature summary for 3.5.1.4 extracted from

  • Maestracci, M.; Thiery, A.; Bui, K.; Arnaud, A.; Galzy, P.
    Activity and regulation of an amidase, acylamide amidohydrolase EC 3.5.1.4, with a wide substrate spectrum from a Brevibacterium sp. (1984), Arch. Microbiol., 138, 315-320.
No PubMed abstract available
Search for more literature for 3.5.1.4

Activating Compound

Activating Compound Comment Organism Structure
acetamide induction of acetamidase Brevibacterium sp.
N-Methylacetamide induction of acetamidase biosynthesis begins at the 3 mM concentration. The induction reaches a maximum plateau between 10 mM and 50 mM Brevibacterium sp.

Inhibitors

Inhibitors Comment Organism Structure
acetic acid
-
 Brevibacterium sp.
Butyric acid
-
 Brevibacterium sp.
N-methylpropionamide amide analogue, inhibits the biosynthesis of acetamidase, strain R312 Brevibacterium sp.
propionic acid
-
 Brevibacterium sp.
Thioacetamide amide analogues, inhibits the biosynthesis of acetamidase, strain R312 Brevibacterium sp.
Valeric acid
-
 Brevibacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.24
-
 Valeramide strain R312 Brevibacterium sp.
0.53
-
 Isobutyramide strain R312 Brevibacterium sp.
1.1
-
 pivalamide strain R312 Brevibacterium sp.
2.5
-
 acetamide strain R312 Brevibacterium sp.
2.7
-
 Benzamide strain R312 Brevibacterium sp.
8
-
 DL-Lactamide strain R312 Brevibacterium sp.
16
-
 Acrylamide mutant strain 19 Brevibacterium sp.
44
-
 Propionamide strain R312 Brevibacterium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
carboxylic acid amide + H2O Brevibacterium sp.
-
 carboxylic acid + NH3
-
 ?
carboxylic acid amide + H2O Brevibacterium sp. R312
-
 carboxylic acid + NH3
-
 ?
DL-lactamide + H2O Brevibacterium sp.
-
 DL-lactate + NH3
-
 ?
DL-lactamide + H2O Brevibacterium sp. R312
-
 DL-lactate + NH3
-
 ?
pivalamide + H2O Brevibacterium sp.
-
 pivalate + NH3
-
 ?
pivalamide + H2O Brevibacterium sp. R312
-
 pivalate + NH3
-
 ?
valeramide + H2O Brevibacterium sp.
-
 valerate + NH3
-
 ?
valeramide + H2O Brevibacterium sp. R312
-
 valerate + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Brevibacterium sp.
-
-
-
Brevibacterium sp. R312
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.3
-
 substrate valeramide, strain R312 Brevibacterium sp.
0.6
-
 substrate pivalamide, strain R312 Brevibacterium sp.
0.8
-
 substrate benzamide, strain R312 Brevibacterium sp.
9
-
 substrate acetamide, strain R312 Brevibacterium sp.
9
-
 substrate isobutyramide, strain R312 Brevibacterium sp.
16
-
 substrate DL-lactamide, strain R312 Brevibacterium sp.
16.7
-
 substrate propionamide, strain R312 Brevibacterium sp.
135
-
 substrate acetamide, strain R312, transfer reaction Brevibacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetamide + H2O
-
 Brevibacterium sp. acetate + NH3
-
 ?
acrylamide + H2O
-
 Brevibacterium sp. acrylate + NH3
-
 ?
benzamide + H2O
-
 Brevibacterium sp. benzoate + NH3
-
 ?
carboxylic acid amide + H2O
-
 Brevibacterium sp. carboxylic acid + NH3
-
 ?
carboxylic acid amide + H2O
-
 Brevibacterium sp. R312 carboxylic acid + NH3
-
 ?
DL-lactamide + H2O
-
 Brevibacterium sp. DL-lactate + NH3
-
 ?
DL-lactamide + H2O
-
 Brevibacterium sp. R312 DL-lactate + NH3
-
 ?
isobutyramide + H2O
-
 Brevibacterium sp. isobutyrate + NH3
-
 ?
isobutyramide + H2O
-
 Brevibacterium sp. R312 isobutyrate + NH3
-
 ?
pivalamide + H2O
-
 Brevibacterium sp. pivalate + NH3
-
 ?
pivalamide + H2O
-
 Brevibacterium sp. R312 pivalate + NH3
-
 ?
propionamide + H2O
-
 Brevibacterium sp. propionate + NH3
-
 ?
valeramide + H2O
-
 Brevibacterium sp. valerate + NH3
-
 ?
valeramide + H2O
-
 Brevibacterium sp. R312 valerate + NH3
-
 ?

Synonyms

Synonyms Comment Organism
acetamidase
-
 Brevibacterium sp.