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Literature summary for 3.5.1.4 extracted from
- Isolation of an inducible amidase from Pseudomonas acidovorans AE1 (1975), J. Gen. Microbiol., 87, 260-272.
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Delftia acidovorans | - |
- |
- |
| Delftia acidovorans AE1 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| lysis of crude extract, addition of streptomycin, ammonium sulfate fractionation, desalting by Sephadex-gel filtration, DEAE-Sepharose chromatography, hydroxyapatite column, yield 72% | Delftia acidovorans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 125 | - |
substrate acetanilide, after disc electrophoresis of purified enzyme | Delftia acidovorans |
| 131 | - |
substrate acetanilide, after purification | Delftia acidovorans |
Storage Stability
| Storage Stability | Organism |
|---|---|
| glycerol 2%, v/v, stabilizes the enzyme, after storing three months at -18°C, 77% of the activity of purified enzyme recovered | Delftia acidovorans |
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