Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DTNB | 15-18% inhibition of wild-type, MM6 and MM8, 35% inhibition of the MM15 activity | Methylophilus methylotrophus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
acetamide | mutant strain MM8 | Methylophilus methylotrophus | |
2 | - |
Acrylamide | mutant strain MM8 | Methylophilus methylotrophus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
4 * 38000 | Methylophilus methylotrophus |
38400 | - |
6 * 38400 | Pseudomonas aeruginosa |
40000 | - |
8 * 40000 | Arthrobacter sp. |
155000 | - |
sedimentation-equilibrium ultracentrifugation | Methylophilus methylotrophus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetamide + H2O | Methylophilus methylotrophus | - |
acetic acid + NH3 | - |
? | |
acylamide + H2O | Methylophilus methylotrophus | - |
carboxylic acid + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter sp. | - |
- |
- |
Methylophilus methylotrophus | - |
wild-type is strain MM6, mutant strains are MM8 and MM15 | - |
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
passage with French pressure cell, cell-free extract purified and concentrated by using anion-exchange and gel filtration, judged by SDS-PAGE and scanning densiometry | Methylophilus methylotrophus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.51 | - |
mutant strain MM15 measured with acrylamide as substrate, growth conditions is continuous | Methylophilus methylotrophus |
2.1 | - |
mutant strain MM15 measured with acetamide as substrate | Methylophilus methylotrophus |
3.59 | - |
mutant strain MM15 measured with acrylamide as substrate, growth conditions is fed-batch | Methylophilus methylotrophus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetamide + H2O | - |
Methylophilus methylotrophus | acetic acid + NH3 | - |
? | |
acrylamide + H2O | - |
Methylophilus methylotrophus | acrylate + NH3 | - |
? | |
acylamide + H2O | - |
Methylophilus methylotrophus | carboxylic acid + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 38400 | Pseudomonas aeruginosa |
octamer | 8 * 40000 | Arthrobacter sp. |
tetramer | 4 * 38000 | Methylophilus methylotrophus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.13 | - |
acetamide | - |
Methylophilus methylotrophus |