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Literature summary for 3.5.1.4 extracted from

  • Ciskanik, L.M.; Wilczek, J.M.; Fallon, R.D.
    Purification and characterization of an enantioselective amidase from Pseudomonas chlororaphis B23 (1995), Appl. Environ. Microbiol., 61, 998-1003.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis acrylamide production Pseudomonas chlororaphis

Inhibitors

Inhibitors Comment Organism Structure
Ag+ preincubation for 30 min, remaining activity in presence of 1 mM DTT Pseudomonas chlororaphis
Cd2+ preincubated for 30 min Pseudomonas chlororaphis
Cu2+ preincubated for 30 min, remaining activity in presence of 1 mM DTT Pseudomonas chlororaphis
DTNB preincubated for 30 min, remaining activity in presence of 1 mM DTT Pseudomonas chlororaphis
Fe2+ preincubated for 30 min Pseudomonas chlororaphis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
2 * 54000, SDS-PAGE Pseudomonas chlororaphis
105000
-
-
Pseudomonas chlororaphis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
propionamide + H2O Pseudomonas chlororaphis
-
propionic acid + NH3
-
?
propionamide + H2O Pseudomonas chlororaphis B23
-
propionic acid + NH3
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas chlororaphis
-
-
-
Pseudomonas chlororaphis B23
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, 30 to 55%, and hydrophobic, anion-exchange, gel filtration, and ceramic hydroxyapatite chromatography steps, DTT required to maintain enzymatic activity, overall level of purification 114-fold, recovery rate 21% Pseudomonas chlororaphis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
17.7
-
turnover propionamide to propionate and ammonia Pseudomonas chlororaphis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
propionamide + H2O
-
Pseudomonas chlororaphis propionic acid + NH3
-
?
propionamide + H2O
-
Pseudomonas chlororaphis B23 propionic acid + NH3
-
?

Subunits

Subunits Comment Organism
dimer 2 * 54000, SDS-PAGE Pseudomonas chlororaphis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
10.5
-
Propionamide
-
Pseudomonas chlororaphis

pH Range

pH Minimum pH Maximum Comment Organism
3.4 11 100% activity at pH values between 5.9 and 9.9 Pseudomonas chlororaphis
7 8.6 against the substrate propionamide Pseudomonas chlororaphis

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.9 9.9 in various buffer Pseudomonas chlororaphis