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Literature summary for 3.5.1.38 extracted from

  • Davidson, L.; Brear, D.R.; Wingard, P.; Hawkins, J.; Kitto, G.B.
    Purification and properties of an L-glutaminase-L-asparaginase from Pseudomonas acidovorans (1977), J. Bacteriol., 129, 1379-1386.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.38

Inhibitors

Inhibitors Comment Organism Structure
p-chloromercuribenzoate 26% inhibition at 0.1 mM Delftia acidovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
 L-asparagine
-
 Delftia acidovorans
0.022
-
 L-glutamine
-
 Delftia acidovorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
 4 * 39000, SDS-PAGE Delftia acidovorans
156000
-
 gel filtration Delftia acidovorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-asparagine + H2O Delftia acidovorans hydrolysed at a rate of 0.83 compared to L-glutamine L-aspartate + NH3
-
 ?
L-glutamine + H2O Delftia acidovorans
-
 L-glutamate + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Delftia acidovorans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, 3step chromatography Delftia acidovorans

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Delftia acidovorans
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
86
-
 asparaginase activity Delftia acidovorans
104
-
 glutaminase activity Delftia acidovorans

Storage Stability

Storage Stability Organism
4°C, 50 mM borate buffer, pH 7.0, 100 mM NaCl, 1 mM EDTA, 3 to 4 months Delftia acidovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-aspartyl hydroxylammonium sulfate + H2O
-
 Delftia acidovorans L-Asp + hydroxylammonium sulfate
-
 ?
beta-L-aspartyl hydroxylammonium sulfate + H2O
-
 Delftia acidovorans L-Asp + hydroxylammonium sulfate
-
 ?
D-asparagine + H2O low activity Delftia acidovorans D-aspartate + NH3
-
 ?
D-glutamine + H2O low activity Delftia acidovorans D-glutamate + NH3
-
 ?
gamma-L-glutamyl hydroxylammonium sulfate + H2O
-
 Delftia acidovorans L-glutamate + hydroxylammonium sulfate
-
 ?
L-asparagine + H2O hydrolysed at a rate of 0.83 compared to L-glutamine Delftia acidovorans L-aspartate + NH3
-
 ?
L-glutamine + H2O
-
 Delftia acidovorans L-glutamate + NH3
-
 ?
N-acetyl-L-asparagine + H2O 5% of the activity compared to L-asparagine Delftia acidovorans N-acetyl-L-aspartate + NH3
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 39000, SDS-PAGE Delftia acidovorans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 half-life: 10 min, protection against thermal inactivation by addition of substrate Delftia acidovorans

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.4
-
 70% activity Delftia acidovorans