Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermococcus kodakarensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
127 | - |
N-acetyl-D-glucosamine | pH 8.5, 75°C | Thermococcus kodakarensis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Thermococcus kodakarensis | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
6 * 30000, SDS-PAGE | Thermococcus kodakarensis |
160000 | - |
gel filtration | Thermococcus kodakarensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-D-glucosamine + H2O | Thermococcus kodakarensis | the enzyme is probably involved in archaeal chitin catabolic pathway | D-glucosamine + acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus kodakarensis | Q6F4N1 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermococcus kodakarensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-D-glucosamine + H2O | - |
Thermococcus kodakarensis | D-glucosamine + acetate | - |
? | |
N-acetyl-D-glucosamine + H2O | the enzyme is probably involved in archaeal chitin catabolic pathway | Thermococcus kodakarensis | D-glucosamine + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homohexamer | 6 * 30000, SDS-PAGE | Thermococcus kodakarensis |
Synonyms | Comment | Organism |
---|---|---|
Tk-Dac | - |
Thermococcus kodakarensis |
TK1764 | locus name | Thermococcus kodakarensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
assay at | Thermococcus kodakarensis |
75 | - |
- |
Thermococcus kodakarensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Thermococcus kodakarensis |
8.5 | - |
- |
Thermococcus kodakarensis |
Organism | Comment | Expression |
---|---|---|
Thermococcus kodakarensis | the transcription is specifically induced by N,N'-diacetylchitobiose, suggesting the function of this gene in chitin catabolism in vivo. N-acetyl-D-glucosamine can not act as an inducer | up |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.53 | - |
N-acetyl-D-glucosamine | pH 8.5, 75°C | Thermococcus kodakarensis |