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Literature summary for 3.5.1.3 extracted from

  • Meister, A.; Levintow, L.; Greenfield, R.E.; Abendschein, P.A.
    Hydrolysis and transfer reactions catalyzed by omega-amidase preparations (1955), J. Biol. Chem., 215, 441-460.
    View publication on PubMed
Search for more literature for 3.5.1.3

Activating Compound

Activating Compound Comment Organism Structure
additional information no activation by ATP, ADP, phosphate or arsenate Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Rattus norvegicus 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no requirement for Mg2+ or Mn2+ for formation of hydroxamate Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-oxoglutaramate + H2O Rattus norvegicus alpha-ketoglutaramate 2-oxoglutarate + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Rattus norvegicus

Reaction

Reaction Comment Organism Reaction ID
a monoamide of a dicarboxylate + H2O = a dicarboxylate + NH3 acyl-enzyme mechanism Rattus norvegicus
a

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Rattus norvegicus
-
liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutaramate + H2O best substrate Rattus norvegicus 2-oxoglutarate + NH3
-
 ?
2-oxoglutaramate + H2O alpha-ketoglutaramate Rattus norvegicus 2-oxoglutarate + NH3
-
 ?
2-oxoglutaramate + hydroxylamine alpha-ketoglutaramate Rattus norvegicus 2-oxoglutaryl hydroxamate + NH3
-
 ?
2-oxosuccinamate + H2O alpha-ketosuccinamate Rattus norvegicus 2-oxosuccinate + NH3
-
 ?
2-oxosuccinamate + H2O no formation of hydroxamate from alpha-ketosuccinamate Rattus norvegicus 2-oxosuccinate + NH3
-
 ?
caproamide + H2O no hydroxamte formation Rattus norvegicus caproic acid + NH3
-
 ?
glutaramate + H2O
-
 Rattus norvegicus glutarate + NH3
-
 ?
glutaramate + hydroxylamine amidase activity, hydrolysis proceeds at a slower rate than formation of glutaryl hydroxamate in the presence of hydroxylamine Rattus norvegicus glutaryl hydroxamate + NH3
-
 ?
glutarate + hydroxylamine at pH 5, not at pH 7 Rattus norvegicus glutaryl hydroxamate
-
 ?
L-isoasparagine + H2O poor substrate Rattus norvegicus L-aspartate + NH3
-
 ?
L-isoglutamine + H2O poor substrate Rattus norvegicus L-glutamate + NH3
-
 ?
L-leucine amide + H2O no hydroxamte formation Rattus norvegicus L-Leu + NH3
-
 ?
L-phenylalanine amide + H2O poor substrate, 12% compared with alpha-ketoglutaramate Rattus norvegicus L-Phe + NH3
-
 ?
additional information substrate specificity Rattus norvegicus ?
-
 ?
additional information catalyzes hydrolysis and transfer reactions, enzyme-dicarboxylic acid complex may react either with H2O or hydroxylamine Rattus norvegicus ?
-
 ?
additional information glutamine, acetamide, propionamide, butyramide, isobutyramide, isovaleramide, oxamate, malonamate, adipamate, oxamide, adipamide, alpha-ketoadipamate, alpha-keto-DL-gamma-methylglutaramate, DL-alpha-aminomalonamate, alpha-methyl-DL-asparagine, DL-gamma-methyl-L-glutamine, alpha-methyl-DL-glutamine, DL-beta-methyl-DL-glutamine, DL-proline, DL-alanine and L-tyrosine amide: no substrates Rattus norvegicus ?
-
 ?
additional information catalyzes hydroxaminolysis and transamidation reactions with esters and amides Rattus norvegicus ?
-
 ?
additional information catalyzes hydrolysis and hydroxaminolysis Rattus norvegicus ?
-
 ?
succinamate + H2O amidase activity, hydrolysis proceeds at a slower rate than formation of succinyl hydroxamate in the presence of hydroxylamine Rattus norvegicus succinic acid + NH3
-
 ?
succinamate + hydroxylamine catalyzes formation of hydroxamate much more rapidly than hydrolysis of the amide Rattus norvegicus succinyl hydroxamate + NH3
-
 r
succinate + hydroxylamine at pH 5, not at pH 7 Rattus norvegicus succinyl hydroxamate + H2O
-
 r
succinyl hydroxamate + H2O
-
 Rattus norvegicus succinate + hydroxylamine
-
 r
succinylmonohydroxamate + H2O
-
 Rattus norvegicus succinate + hydroxylamine
-
 r
valeramide + H2O poor substrate Rattus norvegicus valeric acid + NH3
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
-
 Rattus norvegicus
5
-
 hydroxamate formation, substrate glutarate or succinate Rattus norvegicus
6.5 7.5 broad pH-optimum, substrate glutaramate or succinamate Rattus norvegicus
6.5 7.5 hydroxaminolysis Rattus norvegicus
7
-
 hydrolysis and transfer reactions Rattus norvegicus
7
-
 substrate: succinamate or glutaramate, assay at Rattus norvegicus
8.5 9 pH-optimum for hydroxaminolysis of alpha-ketoglutaramate Rattus norvegicus
9
-
 optimal for hydrolysis of alpha-ketoglutaramate or alpha-ketosuccinamate Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
additional information no requirement for any cofactors for hydroxamate formation Rattus norvegicus