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Literature summary for 3.5.1.29 extracted from

  • McCulloch, K.M.; Mukherjee, T.; Begley, T.P.; Ealick, S.E.
    Structure determination and characterization of the vitamin B6 degradative enzyme (E)-2-(acetamidomethylene)succinate hydrolase (2010), Biochemistry, 49, 1226-1235.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli B834(DE3) cells Mesorhizobium japonicum MAFF 303099

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 9% (w/v) PEG 8000, 200 mM MgCl2, and 100 mM Tris at pH 7.8 Mesorhizobium japonicum MAFF 303099

Protein Variants

Protein Variants Comment Organism
D130N inactive Mesorhizobium japonicum MAFF 303099
S106A inactive Mesorhizobium japonicum MAFF 303099
S230A inactive, the active site mutant cannot be assayed because this protein precipitates in the assay buffer Mesorhizobium japonicum MAFF 303099
S230C inactive Mesorhizobium japonicum MAFF 303099
S230N inactive, the active site mutant cannot be assayed because this protein precipitates in the assay buffer Mesorhizobium japonicum MAFF 303099

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(E)-2-(acetamidomethylene)succinate + H2O Mesorhizobium japonicum MAFF 303099
-
succinic semialdehyde + acetate + CO2 + NH3
-
?

Organism

Organism UniProt Comment Textmining
Mesorhizobium japonicum MAFF 303099
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex 75 gel filtration Mesorhizobium japonicum MAFF 303099

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E)-2-(acetamidomethylene)succinate + H2O
-
Mesorhizobium japonicum MAFF 303099 succinic semialdehyde + acetate + CO2 + NH3
-
?

Synonyms

Synonyms Comment Organism
(E)-2-(acetamidomethylene)succinate hydrolase
-
Mesorhizobium japonicum MAFF 303099
E-2AMS hydrolase
-
Mesorhizobium japonicum MAFF 303099

General Information

General Information Comment Organism
metabolism the enzyme catalyzes the final reaction in the degradation of vitamin B6 Mesorhizobium japonicum MAFF 303099