Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli B834(DE3) cells | Mesorhizobium japonicum MAFF 303099 |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 9% (w/v) PEG 8000, 200 mM MgCl2, and 100 mM Tris at pH 7.8 | Mesorhizobium japonicum MAFF 303099 |
Protein Variants | Comment | Organism |
---|---|---|
D130N | inactive | Mesorhizobium japonicum MAFF 303099 |
S106A | inactive | Mesorhizobium japonicum MAFF 303099 |
S230A | inactive, the active site mutant cannot be assayed because this protein precipitates in the assay buffer | Mesorhizobium japonicum MAFF 303099 |
S230C | inactive | Mesorhizobium japonicum MAFF 303099 |
S230N | inactive, the active site mutant cannot be assayed because this protein precipitates in the assay buffer | Mesorhizobium japonicum MAFF 303099 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E)-2-(acetamidomethylene)succinate + H2O | Mesorhizobium japonicum MAFF 303099 | - |
succinic semialdehyde + acetate + CO2 + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mesorhizobium japonicum MAFF 303099 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Superdex 75 gel filtration | Mesorhizobium japonicum MAFF 303099 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E)-2-(acetamidomethylene)succinate + H2O | - |
Mesorhizobium japonicum MAFF 303099 | succinic semialdehyde + acetate + CO2 + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
(E)-2-(acetamidomethylene)succinate hydrolase | - |
Mesorhizobium japonicum MAFF 303099 |
E-2AMS hydrolase | - |
Mesorhizobium japonicum MAFF 303099 |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme catalyzes the final reaction in the degradation of vitamin B6 | Mesorhizobium japonicum MAFF 303099 |