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Literature summary for 3.5.1.29 extracted from

  • Huynh, M.S.; Snell, E.E.
    Enzymes of vitamin B6 degradation. Purification and properties of two N-acetylamidohydrolases (1985), J. Biol. Chem., 260, 2379-2383.
    View publication on PubMed
Search for more literature for 3.5.1.29

General Stability

General Stability Organism
stable at room temperature between pH 6 and 10 Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
alpha-hydroxyglutarate competitive inhibition Pseudomonas sp.
alpha-ketoglutarate
-
 Arthrobacter sp.
alpha-ketoglutarate competitive inhibition Pseudomonas sp.
citrate competitive inhibition Pseudomonas sp.
diphosphate
-
 Arthrobacter sp.
diphosphate competitive inhibition Pseudomonas sp.
fumarate
-
 Arthrobacter sp.
fumarate competitive inhibition Pseudomonas sp.
glutamate competitive inhibition Pseudomonas sp.
Glutarate competitive inhibition Pseudomonas sp.
Hg2+
-
 Pseudomonas sp.
Itaconate competitively inhibited by di-and tricarboxylic acids, itaconic acid being most effective, monocarboxylic acids do not inhibit Arthrobacter sp.
Itaconate competitively inhibited by di-and tricarboxylic acids, itaconic acid being most effective, monocarboxylic acids do not inhibit Pseudomonas sp.
malate competitive inhibition Pseudomonas sp.
Maleate competitive inhibition Pseudomonas sp.
malonate competitive inhibition Pseudomonas sp.
N-acetyl-DL-aspartate
-
 Arthrobacter sp.
N-acetyl-DL-aspartate competitive inhibition Pseudomonas sp.
oxaloacetate
-
 Arthrobacter sp.
oxaloacetate competitive inhibition Pseudomonas sp.
phosphate
-
 Arthrobacter sp.
phosphate competitive inhibition Pseudomonas sp.
SO32- inhibitory effect increases rapidly with time to a constant level, not competitve with substrate Arthrobacter sp.
SO32- inhibitory effect increases rapidly with time to a constant level, not competitve with substrate Pseudomonas sp.
succinate
-
 Arthrobacter sp.
succinate competitive inhibition Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
 2-(Acetamidomethylene)succinate at pH 6.5 and 25°C Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32500
-
 gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-(acetamidomethylene)succinate + 2 H2O Pseudomonas sp.
-
 acetate + succinate semialdehyde + NH3 + CO2
-
 ?
2-(acetamidomethylene)succinate + 2 H2O Pseudomonas sp. IA
-
 acetate + succinate semialdehyde + NH3 + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Arthrobacter sp.
-
 Cr-7
-
Pseudomonas sp.
-
 MA-1
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.12
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-(acetamidomethylene)succinate + 2 H2O
-
 Pseudomonas sp. acetate + succinate semialdehyde + NH3 + CO2
-
 ?
2-(acetamidomethylene)succinate + 2 H2O
-
 Pseudomonas sp. acetate + succinate semialdehyde + NH3 + CO2 same products as formed by acid hydrolysis ir
2-(acetamidomethylene)succinate + 2 H2O
-
 Pseudomonas sp. IA acetate + succinate semialdehyde + NH3 + CO2
-
 ?
2-(acetamidomethylene)succinate + 2 H2O
-
 Pseudomonas sp. IA acetate + succinate semialdehyde + NH3 + CO2 same products as formed by acid hydrolysis ir

Subunits

Subunits Comment Organism
monomer
-
 Arthrobacter sp.
monomer SDS-PAGE Pseudomonas sp.

Synonyms

Synonyms Comment Organism
compound A hydrolase
-
-

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
 Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70 90 at pH 8 and 70°C stable for 1 min, loses activity completely in 1 min at 90°C Pseudomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.67
-
 2-(Acetamidomethylene)succinate
-
 Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7.5 pH 6.5 in 50 mM sodium phosphate, MES and HEPES buffers, pH 7.5 in Tris buffer Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 10 most efficient between pH 6.0 and 7.0 Pseudomonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 10 loses activity rapidly between 4.0 and 5.0 Pseudomonas sp.