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Literature summary for 3.5.1.28 extracted from

  • Tillman, G.; Simmons, M.; Garrish, J.; Seal, B.
    Expression of a Clostridium perfringens genome-encoded putative N-acetylmuramoyl-L-alanine amidase as a potential antimicrobial to control the bacterium (2013), Arch. Microbiol., 195, 675-681.
No PubMed abstract available
Search for more literature for 3.5.1.28

Cloned(Commentary)

Cloned (Comment) Organism
gene cwlD, DNA and amino acid sequence determination and analysis from strains CP26, CP39, CP509, CP297, CP697, CP726, CP776, CP1036, and CP1113, genotyping, recombinant expression in Escherichia coli strain Rosetta 2 (DE3), cloning in Escherichia coli strain DH5alpha Clostridium perfringens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38969
-
 1 * 40000, SDS-PAGE, 1 * 38969, sequence calculation Clostridium perfringens
40000
-
 gel filtration Clostridium perfringens

Organism

Organism UniProt Comment Textmining
Clostridium perfringens A0A0H2YSJ7 from strain 13124; gene cwlD
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the recombinant enzyme shows lytic activity against Clostridium perfringens cells Clostridium perfringens ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 40000, SDS-PAGE, 1 * 38969, sequence calculation Clostridium perfringens

Synonyms

Synonyms Comment Organism
amidase 3
-
 Clostridium perfringens
MurnAc-lAA
-
 Clostridium perfringens
NAMLA amidase
-
 Clostridium perfringens
NAMLAA
-
 Clostridium perfringens
peptidoglycan amidase
-
 Clostridium perfringens
peptidoglycan aminohydrolase
-
 Clostridium perfringens
PlyCpAmi
-
 Clostridium perfringens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Clostridium perfringens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
 assay at Clostridium perfringens

pI Value

Organism Comment pI Value Maximum pI Value
Clostridium perfringens sequence calculation
-
 8.7

General Information

General Information Comment Organism
physiological function the amidase PlyCpAmi can kill stationary phase cells even though they may have a higher resistance to stress. The enzyme hydrolyzes the amide bond between N-acetylmuramoyl and L-amino acids in certain cell wall glycopeptides Clostridium perfringens