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Literature summary for 3.5.1.28 extracted from

  • Park, J.; Yun, J.; Lim, J.A.; Kang, D.H.; Ryu, S.
    Characterization of an endolysin, LysBPS13, from a Bacillus cereus bacteriophage (2012), FEMS Microbiol. Lett., 332, 76-83.
    View publication on PubMed
Search for more literature for 3.5.1.28

Activating Compound

Activating Compound Comment Organism Structure
NaCl the highest lytic activity is observed in the presences of 250 mM NaCl Bacillus cereus phage

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Star(DE3) cells Bacillus cereus phage

Inhibitors

Inhibitors Comment Organism Structure
CTAB almost complete inhibition at 0.1 (v/v) Bacillus cereus phage
additional information the activity of the enzyme is not inhibited by 300 mM EDTA or CHAPS, Triton X-100 and Tween-20 (0.1% (v/v) each) Bacillus cereus phage
SDS almost complete inhibition at 0.1 (v/v) Bacillus cereus phage

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme needs no metal ions for its lytic activity, 1 mM MgCl2, CaCl2, ZnCl2, or MnCl2 does not affect lytic activity Bacillus cereus phage
Zn2+ the enzyme contains three zinc-binding sites Bacillus cereus phage

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 x * 35000, SDS-PAGE Bacillus cereus phage

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
peptidoglycan + H2O Bacillus cereus phage
-
 ?
-
 ?
peptidoglycan + H2O Bacillus cereus phage BPS13
-
 ?
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
Triton X-100 about 130% activity in the presence of 0.1 (v/v) Triton X-100 Bacillus cereus phage
Tween 20 about 115% activity in the presence of 0.1 (v/v) Tween 20 Bacillus cereus phage

Organism

Organism UniProt Comment Textmining
Bacillus cereus phage
-
-
-
Bacillus cereus phage BPS13
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography Bacillus cereus phage

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetylmuramoyl-L-alanine + H2O
-
 Bacillus cereus phage N-acetylmuramate + L-alanine
-
 ?
N-acetylmuramoyl-L-alanine + H2O
-
 Bacillus cereus phage BPS13 N-acetylmuramate + L-alanine
-
 ?
peptidoglycan + H2O
-
 Bacillus cereus phage ?
-
 ?
peptidoglycan + H2O
-
 Bacillus cereus phage BPS13 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 35000, SDS-PAGE Bacillus cereus phage

Synonyms

Synonyms Comment Organism
endolysin
-
 Bacillus cereus phage
LysBPS13
-
 Bacillus cereus phage
N-acetylmuramyl-L-alanine amidase
-
 Bacillus cereus phage

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
42 45
-
 Bacillus cereus phage

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 55 about 50% activity at 35 and 55°C Bacillus cereus phage

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 100 the enzyme demonstrates remarkable thermostability in the presence of 30% (v/v) glycerol, and it retains its lytic activity after incubation between 4 and 100°C for 30 min Bacillus cereus phage

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
 Bacillus cereus phage

pH Range

pH Minimum pH Maximum Comment Organism
7.5 10.5 lytic activity significantly decreases at above pH 10.5 and below pH 7.5 Bacillus cereus phage

General Information

General Information Comment Organism
physiological function endolysin LysBPS13 demonstrates high lytic activity against Bacillus cereus Bacillus cereus phage